Detailed information of TA system TA227824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 5101263..5101876
• Replicon: chromosome
• Accession: NZ_CP089216
• Organism: Mycobacterium kansasii strain FDAARGOS_1615

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7ZK47
• Locus tag: LTS70_RS22435
• Protein ID: WP_023365532.1
• Coordinates: 5101263..5101652 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTS70_RS22440
• Protein ID: WP_036393214.1
• Coordinates: 5101649..5101876 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS70_RS22415 (LTS70_22415)	5096502..5097212	+	711	WP_023365512.1	hypothetical protein	-
LTS70_RS22420 (LTS70_22420)	5098393..5098650	+	258	WP_099184175.1	hypothetical protein	-
LTS70_RS22430 (LTS70_22430)	5100000..5100244	-	245	Protein_4442	tyrosine-type recombinase/integrase	-
LTS70_RS22435 (LTS70_22435)	5101263..5101652	-	390	WP_023365532.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTS70_RS22440 (LTS70_22440)	5101649..5101876	-	228	WP_036393214.1	hypothetical protein	Antitoxin
LTS70_RS22445 (LTS70_22445)	5102212..5104842	+	2631	WP_023365536.1	cation-transporting P-type ATPase	-
LTS70_RS22450 (LTS70_22450)	5104887..5105114	-	228	WP_023365538.1	hypothetical protein	-
LTS70_RS22455 (LTS70_22455)	5105137..5105517	-	381	WP_042313314.1	DUF732 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5090287..5121222	30935

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-116)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13970.16 Da
• Isoelectric Point: 7.0619

>T227824 WP_023365532.1 NZ_CP089216:c5101652-5101263 [Mycobacterium kansasii]
VIVLDASAAVELVLATPPGAAVARRLRDETVHAPAHIDVEVIGAIRRAVVRRLISDHEGVVAVADFLSLQIRRWPIKPFA
QRAYQLRTTHIVADAVYVALAEGFGVRLITCDGRLAQSHGHDAEIELVA

Nucleotide

• Length: 390 bp

>T227824 NZ_CP123046:c4174391-4174020 [Escherichia coli]
ATGCAAACTATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGCGGTTACTGAC
TTGTCTGTTGTCGCAGCACTATGGTCTGACGTTGAATGACACACCGTTCAGTAATGAAACCACCATACTGGAACACATTG
ATGCTGGAGTATCGCTGTGCGATGCGGTGAACTTTCTTGTTGAAAAATATGAACTGGTAAGAATTGACTGTAATGATTGC
TCTGTCATGGAGAAGTCACCGTTCATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACGCAA
CAGTCACAAAACTGTTACCAGGGTAACGGCAGGTCGACTTCAGGAGCTATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8173.38 Da
• Isoelectric Point: 4.7513

>AT227824 WP_036393214.1 NZ_CP089216:c5101876-5101649 [Mycobacterium kansasii]
MSTMVQIRNVPDELVHELKARAAAQRMSLSDFLLARLAEFAQEPALDEVLGRLAALPRRDLGASAAELVGEARSE

Nucleotide

• Length: 228 bp

>AT227824 NZ_CP123046:c4174795-4174430 [Escherichia coli]
ATGAACAATCACTCAGAATCCGGTACAAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTTAAATGTGCTATTACCCC
CTGTTTTGGTGCCCGCCTGGTACAGGAGGGCAACCGATTGCATTTTCTCTCTGACCGCGCAGGATTTAGCGGGGCATTTG
CCGTCGATGTTGCAATGCGCCTTGACCAGGCATTTCCGTTAATGATGAAGCAACTTGAGCTGATGCTGACCAGTGGAGAG
CTTAACCCTCGCCACCCACACTGCGTCACGCTTTACCACAATGGACTCACCTGTGAAGCCGATACACTAGGCTCCTGTGG
TTATGTCTACATTGCTATCTATCCTGAGCAAACTGAGCCGCAGTAA

Structures

Toxin

• AlphaFold DB: X7ZK47