Detailed information of TA system TA227823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3457535..3458220
• Replicon: chromosome
• Accession: NZ_CP089216
• Organism: Mycobacterium kansasii strain FDAARGOS_1615

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7ZUB0
• Locus tag: LTS70_RS15420
• Protein ID: WP_023373049.1
• Coordinates: 3457810..3458220 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTS70_RS15415
• Protein ID: WP_036392171.1
• Coordinates: 3457535..3457813 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS70_RS15395 (LTS70_15395)	3454478..3454990	+	513	WP_036391447.1	Holliday junction resolvase RuvX	-
LTS70_RS15400 (LTS70_15400)	3454983..3456230	+	1248	WP_023373040.1	endolytic transglycosylase MltG	-
LTS70_RS15405 (LTS70_15405)	3456208..3457032	+	825	WP_023373043.1	shikimate dehydrogenase	-
LTS70_RS15410 (LTS70_15410)	3457044..3457472	+	429	WP_023373045.1	A24 family peptidase	-
LTS70_RS15415 (LTS70_15415)	3457535..3457813	+	279	WP_036392171.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LTS70_RS15420 (LTS70_15420)	3457810..3458220	+	411	WP_023373049.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTS70_RS15425 (LTS70_15425)	3458349..3459554	+	1206	WP_023373051.1	chorismate synthase	-
LTS70_RS15430 (LTS70_15430)	3459571..3460107	+	537	WP_023373053.1	shikimate kinase	-
LTS70_RS15435 (LTS70_15435)	3460104..3461192	+	1089	WP_023373055.1	3-dehydroquinate synthase	-
LTS70_RS15440 (LTS70_15440)	3461189..3461629	+	441	WP_023373057.1	type II 3-dehydroquinate dehydratase	-
LTS70_RS15445 (LTS70_15445)	3461626..3462378	-	753	WP_036448656.1	B-4DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-48)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14705.90 Da
• Isoelectric Point: 4.8944

>T227823 WP_023373049.1 NZ_CP089216:3457810-3458220 [Mycobacterium kansasii]
VIYLDTSALTKLLIAEPETDELRNWLTVHTDQGEFAATSALSRVELMRVVARQGESGQAERARYLLDGLDILPLTAPVIN
LAETIGPATLRTLDAIHLAAATQIKRELTAFVTYDHRLLAGCLEVGLVTASPGAVR

Nucleotide

• Length: 411 bp

>T227823 NZ_CP123046:3781023-3781301 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10114.45 Da
• Isoelectric Point: 4.8604

>AT227823 WP_036392171.1 NZ_CP089216:3457535-3457813 [Mycobacterium kansasii]
VEAIGVRELRQHASRYLARVEAGEELGITNNGRLVARLVPVQAAERSREALIESGTLIPAPCPQNLLDVTATPAPDRKRT
LSDVLDEMRDEQ

Nucleotide

• Length: 279 bp

>AT227823 NZ_CP123046:3780760-3781020 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: X7ZUB0