Detailed information of TA system TA022782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1756761..1757446
• Replicon: chromosome
• Accession: NC_010551
• Organism: Burkholderia ambifaria MC40-6

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1YQB5
• Locus tag: BAMMC406_RS07985
• Protein ID: WP_012363863.1
• Coordinates: 1757024..1757446 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B1YQB4
• Locus tag: BAMMC406_RS07980
• Protein ID: WP_012363862.1
• Coordinates: 1756761..1757027 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMMC406_RS07965	1752254..1754539	+	2286	WP_175775012.1	TonB-dependent siderophore receptor	-
BAMMC406_RS07970	1754626..1755465	+	840	WP_012363860.1	folate-dependent phosphoribosylglycinamide formyltransferase PurN-like protein	-
BAMMC406_RS07975	1755465..1756481	+	1017	WP_012363861.1	acetyltransferase	-
BAMMC406_RS07980	1756761..1757027	+	267	WP_012363862.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BAMMC406_RS07985	1757024..1757446	+	423	WP_012363863.1	type II toxin-antitoxin system VapC family toxin	Toxin
BAMMC406_RS07990	1757489..1758394	-	906	WP_012363864.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BAMMC406_RS07995	1758363..1759433	-	1071	WP_012363865.1	hypothetical protein	-
BAMMC406_RS08000	1759789..1760952	+	1164	WP_012363866.1	amidohydrolase	-
BAMMC406_RS08005	1760995..1762299	-	1305	WP_012363867.1	cobyrinate a,c-diamide synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14774.90 Da
• Isoelectric Point: 7.8887

>T22782 WP_012363863.1 NC_010551:1757024-1757446 [Burkholderia ambifaria MC40-6]
VTTLYMLDTNICSFIMRERPSAVLSRLQSCVNAQHRIVVSAITYAEMRFGAVGKKASPKHAELVTAFVSRLDGVLSWDTA
AVDATTAIRVDLAARGTPIGANDASIAGHAIAAGAVLVTNNSREFSRVAGLSLEDWAASV

Nucleotide

• Length: 423 bp

>T22782 NC_010551:1757024-1757446 [Burkholderia ambifaria MC40-6]
GTGACGACGCTCTACATGCTTGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCATCCGCGGTGTTGTCCCGGTT
GCAGTCGTGCGTCAATGCGCAGCACCGGATCGTCGTGTCGGCGATCACCTATGCCGAAATGCGGTTCGGCGCAGTCGGCA
AAAAGGCTTCGCCGAAGCATGCGGAACTCGTGACGGCCTTTGTCTCGCGGCTGGATGGCGTGCTGTCGTGGGATACCGCG
GCGGTCGATGCAACGACCGCGATTCGCGTCGACCTGGCCGCACGTGGCACGCCGATCGGCGCGAACGACGCGTCGATCGC
CGGTCACGCGATCGCGGCCGGTGCCGTGCTTGTAACGAACAACAGTCGCGAATTCAGCCGTGTCGCGGGGTTGTCGCTCG
AAGACTGGGCAGCCTCGGTCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10038.27 Da
• Isoelectric Point: 4.5626

>AT22782 WP_012363862.1 NC_010551:1756761-1757027 [Burkholderia ambifaria MC40-6]
MRTVSIFKNARNQAIRIPKDMEFEGVTELEIRREGDTLLLRPVRPTWLSFASEPHADADFLVERPAVVEPGRFDLPGADD
DARTESDR

Nucleotide

• Length: 267 bp

>AT22782 NC_010551:1756761-1757027 [Burkholderia ambifaria MC40-6]
ATGCGCACCGTTTCCATTTTCAAGAACGCCCGTAACCAGGCGATCCGCATCCCGAAGGACATGGAGTTCGAGGGTGTGAC
GGAACTCGAGATCCGCCGCGAGGGCGACACGTTGCTGCTGCGTCCGGTGCGGCCGACGTGGCTGTCGTTCGCGAGTGAAC
CACACGCCGATGCCGACTTCCTCGTCGAGCGCCCGGCGGTTGTCGAACCTGGTCGTTTCGACCTGCCCGGCGCCGATGAC
GATGCACGGACGGAGTCGGACCGGTGA

Structures

Toxin

• AlphaFold DB: B1YQB5

Antitoxin

• AlphaFold DB: B1YQB4