Detailed information of TA system TA227819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1604862..1605550
• Replicon: chromosome
• Accession: NZ_CP089216
• Organism: Mycobacterium kansasii strain FDAARGOS_1615

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7Z9W3
• Locus tag: LTS70_RS07225
• Protein ID: WP_023370286.1
• Coordinates: 1604862..1605293 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LTS70_RS07230
• Protein ID: WP_036394775.1
• Coordinates: 1605290..1605550 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTS70_RS07190 (LTS70_07190)	1600392..1600700	+	309	WP_023370272.1	DUF3263 domain-containing protein	-
LTS70_RS07195 (LTS70_07195)	1600765..1601235	+	471	WP_036394685.1	LytR C-terminal domain-containing protein	-
LTS70_RS07200 (LTS70_07200)	1601354..1602070	+	717	WP_023370276.1	superoxide dismutase family protein	-
LTS70_RS07205 (LTS70_07205)	1602051..1603214	+	1164	WP_103797770.1	glutamate--cysteine ligase	-
LTS70_RS07210 (LTS70_07210)	1603297..1603698	+	402	WP_103797769.1	hypothetical protein	-
LTS70_RS07215 (LTS70_07215)	1603770..1604009	+	240	WP_155254706.1	hypothetical protein	-
LTS70_RS07220 (LTS70_07220)	1604206..1604853	+	648	WP_023370284.1	LON peptidase substrate-binding domain-containing protein	-
LTS70_RS07225 (LTS70_07225)	1604862..1605293	-	432	WP_023370286.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTS70_RS07230 (LTS70_07230)	1605290..1605550	-	261	WP_036394775.1	CopG family transcriptional regulator	Antitoxin
LTS70_RS07235 (LTS70_07235)	1605595..1607799	-	2205	WP_023370290.1	AAA family ATPase	-
LTS70_RS07240 (LTS70_07240)	1607796..1608656	-	861	WP_023370292.1	CDP-diacylglycerol--serine O-phosphatidyltransferase	-
LTS70_RS07245 (LTS70_07245)	1608653..1609348	-	696	WP_023370294.1	phosphatidylserine decarboxylase	-
LTS70_RS07250 (LTS70_07250)	1609469..1609718	-	250	Protein_1429	molybdopterin molybdenumtransferase MoeA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-134)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-40)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15954.13 Da
• Isoelectric Point: 5.0701

>T227819 WP_023370286.1 NZ_CP089216:c1605293-1604862 [Mycobacterium kansasii]
VIIPDVNLLLYAVITGFPQHRRAYAWWHDTVNSQERIGLTYPAVFGFLRIVTNARILTEPLSATEAVAYVRDWLTQPNVD
LLIPSARHLDLALGLLEQLGTASNLTTDVQLAAYAIEHNAEMHSSDADFARFAGLAWTNPLDR

Nucleotide

• Length: 432 bp

>T227819 NZ_CP123046:2356088-2357410 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10093.61 Da
• Isoelectric Point: 9.9864

>AT227819 WP_036394775.1 NZ_CP089216:c1605550-1605290 [Mycobacterium kansasii]
MRTTLTLDDDVVRLIEEAVHRERRPMKHVINDALRKALAQPMERRTPYQLKPHRSAVRPGFDFAGFNRLADEMEDQAILD
QKRPTL

Nucleotide

• Length: 261 bp

>AT227819 NZ_CP123046:2355822-2356088 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: X7Z9W3