Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 6446587..6447128 Replicon chromosome
Accession NZ_CP089215
Organism Mycolicibacterium smegmatis strain FDAARGOS_1618

Toxin (Protein)


Gene name mazF Uniprot ID A0A2U9PVI3
Locus tag LTT02_RS30945 Protein ID WP_003895797.1
Coordinates 6446587..6446916 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0R0N3
Locus tag LTT02_RS30950 Protein ID WP_011729843.1
Coordinates 6446919..6447128 (-) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LTT02_RS30920 (LTT02_30920) 6442634..6443014 - 381 WP_174519608.1 phage holin family protein -
LTT02_RS30930 (LTT02_30930) 6443231..6444943 - 1713 WP_058126565.1 NAD(P)/FAD-dependent oxidoreductase -
LTT02_RS30935 (LTT02_30935) 6444940..6445875 - 936 WP_003895799.1 alpha/beta fold hydrolase -
LTT02_RS30940 (LTT02_30940) 6445981..6446544 + 564 WP_014878038.1 helix-turn-helix domain-containing protein -
LTT02_RS30945 (LTT02_30945) 6446587..6446916 - 330 WP_003895797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LTT02_RS30950 (LTT02_30950) 6446919..6447128 - 210 WP_011729843.1 antitoxin MazE family protein Antitoxin
LTT02_RS30955 (LTT02_30955) 6447289..6448656 - 1368 WP_081319552.1 dihydrolipoyl dehydrogenase -
LTT02_RS30960 (LTT02_30960) 6448918..6449202 + 285 WP_011729841.1 hypothetical protein -
LTT02_RS30965 (LTT02_30965) 6449199..6450119 + 921 WP_011729840.1 NADP-dependent oxidoreductase -
LTT02_RS30970 (LTT02_30970) 6450149..6450550 + 402 Protein_6144 cupin domain-containing protein -
LTT02_RS30975 (LTT02_30975) 6451087..6451857 + 771 WP_011729837.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11739.52 Da        Isoelectric Point: 10.4023

>T227818 WP_003895797.1 NZ_CP089215:c6446916-6446587 [Mycolicibacterium smegmatis]
VRRGDIYTAAARGAYTGKPRPVLIIQDDRFDATASVTVVPFTTSDTDAPLLRIRIEPTPATGLSTVSSLMIDKVTTVPRS
SLTHRVGRLAETDMVRTDRALLVFLGIAG

Download         Length: 330 bp

>T227818 NZ_CP123046:2034790-2034893 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8114.98 Da        Isoelectric Point: 6.2231

>AT227818 WP_011729843.1 NZ_CP089215:c6447128-6446919 [Mycolicibacterium smegmatis]
MTPARDRVRRHRERLRRQGLRPVQIWVPDVNAPEFRREAHRQSELVAAGEHEAEDQAFVDAISVDWDDA

Download         Length: 210 bp

>AT227818 NZ_CP123046:c2034931-2034660 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGTAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PVI3

Antitoxin

Source ID Structure
AlphaFold DB A0R0N3

References