Detailed information of TA system TA227817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5245268..5245909
• Replicon: chromosome
• Accession: NZ_CP089215
• Organism: Mycolicibacterium smegmatis strain FDAARGOS_1618

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LTT02_RS25245
• Protein ID: WP_011730672.1
• Coordinates: 5245466..5245909 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A2U9PXD6
• Locus tag: LTT02_RS25240
• Protein ID: WP_003897042.1
• Coordinates: 5245268..5245456 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT02_RS25230 (LTT02_25230)	5241246..5242853	+	1608	WP_162267879.1	serine hydrolase	-
LTT02_RS25235 (LTT02_25235)	5242867..5245242	+	2376	WP_058126983.1	cation-translocating P-type ATPase	-
LTT02_RS25240 (LTT02_25240)	5245268..5245456	+	189	WP_003897042.1	antitoxin	Antitoxin
LTT02_RS25245 (LTT02_25245)	5245466..5245909	+	444	WP_011730672.1	SRPBCC family protein	Toxin
LTT02_RS25250 (LTT02_25250)	5245914..5247386	-	1473	WP_058127741.1	long-chain fatty acid--CoA ligase	-
LTT02_RS25255 (LTT02_25255)	5247383..5248156	-	774	WP_011730670.1	SDR family oxidoreductase	-
LTT02_RS25260 (LTT02_25260)	5248157..5249689	-	1533	WP_058126982.1	cyclohexanecarboxylate-CoA ligase	-
LTT02_RS25265 (LTT02_25265)	5249691..5250428	-	738	WP_011730668.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16157.75 Da
• Isoelectric Point: 9.9432

>T227817 WP_011730672.1 NZ_CP089215:5245466-5245909 [Mycolicibacterium smegmatis]
MAKLSVSVEVPLPPEQAWEYASDLSRYHEWLSIHRAWRSKLPETLEKGTVIESIVEVKGMLNRVRWTLVHYKPPQALTLN
GDGRGGVKVKLIGKIKPTDNGATVGFDLHLGGPALFGPIGMVVAAALKSDIQESLNRFKQLYAPSAT

Nucleotide

• Length: 444 bp

>T227817 NZ_CP123046:1825304-1825558 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6886.64 Da
• Isoelectric Point: 4.6906

>AT227817 WP_003897042.1 NZ_CP089215:5245268-5245456 [Mycolicibacterium smegmatis]
MGFLDKAKDLLSQNADKVDTVIDKAGDMIDEKTQGKYAQHVDKAQDAAKNAIRKDDPQEPQQ

Nucleotide

• Length: 189 bp

>AT227817 NZ_CP123046:1825056-1825307 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATAGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U9PXD6