Detailed information of TA system TA227814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2650573..2651216
• Replicon: chromosome
• Accession: NZ_CP089215
• Organism: Mycolicibacterium smegmatis strain FDAARGOS_1618

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LTT02_RS12870
• Protein ID: WP_058125487.1
• Coordinates: 2650573..2650962 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0QRY5
• Locus tag: LTT02_RS12875
• Protein ID: WP_003892665.1
• Coordinates: 2650962..2651216 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT02_RS12855 (LTT02_12855)	2645933..2646370	+	438	WP_058125490.1	SRPBCC family protein	-
LTT02_RS12860 (LTT02_12860)	2646367..2648127	+	1761	WP_058125489.1	sulfatase-like hydrolase/transferase	-
LTT02_RS12865 (LTT02_12865)	2648149..2650554	+	2406	WP_058125488.1	SEC-C metal-binding domain-containing protein	-
LTT02_RS12870 (LTT02_12870)	2650573..2650962	-	390	WP_058125487.1	type II toxin-antitoxin system VapC family toxin	Toxin
LTT02_RS12875 (LTT02_12875)	2650962..2651216	-	255	WP_003892665.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LTT02_RS12880 (LTT02_12880)	2651237..2652406	-	1170	WP_058125486.1	DUF3322 and DUF2220 domain-containing protein	-
LTT02_RS12885 (LTT02_12885)	2652403..2655786	-	3384	WP_058125485.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13894.67 Da
• Isoelectric Point: 4.4731

>T227814 WP_058125487.1 NZ_CP089215:c2650962-2650573 [Mycolicibacterium smegmatis]
MVIDTSALVAILTDEPDAELLEGAVADDPVRTMSTASYLETAIVIESRFGEPGGRELDLWLHRASVALVAVDADQADAAR
LAYRRYGKGRHPAGLNYGDCFSYALAKVSGQPLLFKGEDFRLTDVAAVH

Nucleotide

• Length: 390 bp

>T227814 NZ_CP123046:1040394-1040729 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9279.72 Da
• Isoelectric Point: 8.0238

>AT227814 WP_003892665.1 NZ_CP089215:c2651216-2650962 [Mycolicibacterium smegmatis]
MALSIKHPEADRLARELAARTGETLTEAVVMALRERLARTVGRTQVVPLREELAAIRRRCAALPVLDDRTAESILGYDDR
GLPS

Nucleotide

• Length: 255 bp

>AT227814 NZ_CP123046:1040146-1040394 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0QRY5