Detailed information of TA system TA227813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 515587..516583
• Replicon: chromosome
• Accession: NZ_CP089215
• Organism: Mycolicibacterium smegmatis strain FDAARGOS_1618

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: LTT02_RS02455
• Protein ID: WP_058126279.1
• Coordinates: 515587..516165 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: I7GB97
• Locus tag: LTT02_RS02460
• Protein ID: WP_003894879.1
• Coordinates: 516191..516583 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT02_RS02435 (LTT02_02435)	511545..512105	+	561	WP_058126281.1	hypothetical protein	-
LTT02_RS02440 (LTT02_02440)	512139..513527	-	1389	WP_058126280.1	L-serine ammonia-lyase	-
LTT02_RS02445 (LTT02_02445)	513733..513888	-	156	WP_003894882.1	hypothetical protein	-
LTT02_RS02450 (LTT02_02450)	514223..515575	+	1353	WP_011729067.1	alpha/beta hydrolase-fold protein	-
LTT02_RS02455 (LTT02_02455)	515587..516165	-	579	WP_058126279.1	zeta toxin family protein	Toxin
LTT02_RS02460 (LTT02_02460)	516191..516583	-	393	WP_003894879.1	ParD-like family protein	Antitoxin
LTT02_RS02465 (LTT02_02465)	516647..517276	-	630	WP_003894878.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
LTT02_RS02470 (LTT02_02470)	517586..519214	+	1629	WP_058126278.1	GMC family oxidoreductase N-terminal domain-containing protein	-
LTT02_RS02475 (LTT02_02475)	519216..519821	-	606	WP_003894876.1	class I SAM-dependent methyltransferase	-
LTT02_RS02480 (LTT02_02480)	519995..521113	-	1119	WP_003894875.1	acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21118.31 Da
• Isoelectric Point: 9.9425

>T227813 WP_058126279.1 NZ_CP089215:c516165-515587 [Mycolicibacterium smegmatis]
VKRLDLVVGPNGAGKSTFVELTLAPLLTGSAFVNADEIAKRRWPHAPAEHSYDAARIASDTRLKLIELGESFIAETVFSH
PSKLQLIDTAQAAGYTVVMHVVMIPEDLAVLRVQHRVRAGGHHVPEEKIRERHQRLWPLVALAAARADISTFYDNSAVRG
PRIVAQLAGGFAVGRPAWPMWAPQVLTSRWPE

Nucleotide

• Length: 579 bp

>T227813 NZ_CP123046:902549-902956 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 13841.29 Da
• Isoelectric Point: 4.9246

>AT227813 WP_003894879.1 NZ_CP089215:c516583-516191 [Mycolicibacterium smegmatis]
MTETADRVTRFAADLVESAAAEGARQSRSAKQQLDHWARVGRAVSSQHTAARRRVEAALAGDLALRDLTPEEGVVFNAEI
SAAIEENLAHTDYGQVLAGRGVTTVALDDDGAIVRYQPDGTTTVLAPAQR

Nucleotide

• Length: 393 bp

>AT227813 NZ_CP123046:902302-902568 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U9PRP8