Detailed information of TA system TA227812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-YefM
• Location: 5122640..5123202
• Replicon: chromosome
• Accession: NZ_CP089214
• Organism: Nocardia asteroides strain FDAARGOS_1620

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LTT61_RS23760
• Protein ID: WP_233016265.1
• Coordinates: 5122640..5122888 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LTT61_RS23765
• Protein ID: WP_233016266.1
• Coordinates: 5122918..5123202 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTT61_RS23725 (LTT61_23725)	5117720..5118511	+	792	WP_269821790.1	enoyl-CoA hydratase	-
LTT61_RS23730 (LTT61_23730)	5118692..5119783	-	1092	WP_233016260.1	hypothetical protein	-
LTT61_RS23735 (LTT61_23735)	5119852..5120418	-	567	WP_233016261.1	MarR family transcriptional regulator	-
LTT61_RS23745 (LTT61_23745)	5120606..5121007	+	402	WP_233016262.1	Imm7 family immunity protein	-
LTT61_RS23750 (LTT61_23750)	5121042..5121821	-	780	WP_233016263.1	hypothetical protein	-
LTT61_RS23755 (LTT61_23755)	5121998..5122537	+	540	WP_233016264.1	orotate phosphoribosyltransferase	-
LTT61_RS23760 (LTT61_23760)	5122640..5122888	-	249	WP_233016265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LTT61_RS23765 (LTT61_23765)	5122918..5123202	-	285	WP_233016266.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LTT61_RS23770 (LTT61_23770)	5123295..5123465	-	171	WP_233016267.1	hypothetical protein	-
LTT61_RS23775 (LTT61_23775)	5123679..5124866	-	1188	WP_233016268.1	helix-turn-helix domain-containing protein	-
LTT61_RS23785 (LTT61_23785)	5125096..5125764	-	669	WP_233021165.1	DUF305 domain-containing protein	-
LTT61_RS23790 (LTT61_23790)	5125815..5126726	-	912	WP_233016269.1	DUF3105 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (19-80)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9458.72 Da
• Isoelectric Point: 10.6125

>T227812 WP_233016265.1 NZ_CP089214:c5122888-5122640 [Nocardia asteroides]
MSAQARRNIHENLPLDVALAAMEAISGPIATNPHRVGKPLNEPFDGFYSARRGTYRIVYRINEDKRSVEIHSVRHRRDVY
RT

Nucleotide

• Length: 249 bp

>T227812 NZ_CP123046:c599633-599169 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGTGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCTTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10804.10 Da
• Isoelectric Point: 4.5348

>AT227812 WP_233016266.1 NZ_CP089214:c5123202-5122918 [Nocardia asteroides]
MEIIPITEAKARIAELADRVAREHDHFTITRNGRADVMLISVVEYESMQETLEILSDRDTLADLRQSREDFASGETFDVD
QVRAELEKRRRGVA

Nucleotide

• Length: 285 bp

>AT227812 NZ_CP123046:c599968-599633 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA