Detailed information of TA system TA022780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/PemK(toxin)
• Location: 150098..150672
• Replicon: chromosome
• Accession: NC_010547
• Organism: Cyanothece sp. ATCC 51142

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: B1X2A8
• Locus tag: CCE_RS23170
• Protein ID: WP_009546320.1
• Coordinates: 150098..150442 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: B1X2A9
• Locus tag: CCE_RS23175
• Protein ID: WP_009546319.1
• Coordinates: 150436..150672 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCE_RS23145	145399..145827	+	429	WP_009546325.1	hypothetical protein	-
CCE_RS23150	145832..146206	+	375	WP_009546324.1	HNH endonuclease	-
CCE_RS23155	146381..147700	+	1320	WP_009546323.1	hypothetical protein	-
CCE_RS23160	147904..148923	+	1020	WP_009546322.1	GIY-YIG nuclease family protein	-
CCE_RS23165	148884..149834	-	951	WP_009546321.1	tyrosine-type recombinase/integrase	-
CCE_RS23170	150098..150442	-	345	WP_009546320.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CCE_RS23175	150436..150672	-	237	WP_009546319.1	hypothetical protein	Antitoxin
CCE_RS23180	150747..151391	-	645	WP_009546318.1	hypothetical protein	-
CCE_RS23185	151457..152002	+	546	WP_009546317.1	Uma2 family endonuclease	-
CCE_RS23190	152286..153731	-	1446	WP_009546316.1	carotenoid oxygenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12803.74 Da
• Isoelectric Point: 4.7377

>T22780 WP_009546320.1 NC_010547:c150442-150098 [Crocosphaera subtropica ATCC 51142]
MVIHRGDIWWANLPSPVGSEPGYRRPILVVQDDGFNRSQIQTVIAVILTSNLNLSQAPGNVYLPRNQTSLPKDSVVNVSQ
IVTIDKAFLTEKITCLDEDLMERIDEGLRLVLYL

Nucleotide

• Length: 345 bp

>T22780 NC_010547:c150442-150098 [Crocosphaera subtropica ATCC 51142]
ATGGTGATTCATCGAGGGGATATTTGGTGGGCTAATCTGCCCTCTCCTGTAGGGAGTGAACCTGGATATCGTCGTCCTAT
TTTAGTGGTTCAAGATGATGGGTTTAACCGCAGTCAAATTCAAACCGTCATTGCTGTTATTTTGACCTCAAATCTCAACT
TGAGCCAAGCTCCAGGCAATGTTTATTTACCAAGAAATCAAACCTCACTACCAAAAGATTCAGTAGTGAATGTTTCACAA
ATTGTCACTATTGATAAAGCTTTTTTAACTGAAAAAATAACTTGTTTAGATGAAGATTTGATGGAAAGGATCGATGAAGG
ATTACGTTTGGTTTTGTATCTTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8987.10 Da
• Isoelectric Point: 5.0190

>AT22780 WP_009546319.1 NC_010547:c150672-150436 [Crocosphaera subtropica ATCC 51142]
VKTAISIPDDIFTEAEKLAQKLHISRSELYTKAITAYLKNSRSSQITEKLNQVYQDTDSQLPQDITQMQLSSIEKEQW

Nucleotide

• Length: 237 bp

>AT22780 NC_010547:c150672-150436 [Crocosphaera subtropica ATCC 51142]
GTGAAAACAGCAATTTCAATTCCTGATGACATTTTTACAGAAGCAGAAAAGTTAGCTCAAAAACTTCATATTAGTCGAAG
TGAACTTTATACTAAAGCCATTACTGCTTATCTAAAAAATTCTCGTTCTTCTCAAATCACGGAAAAGCTTAATCAAGTTT
ATCAAGATACAGATAGTCAACTGCCCCAAGACATTACACAAATGCAATTGAGTTCGATAGAGAAAGAACAATGGTGA

Structures

Toxin

• AlphaFold DB: B1X2A8

Antitoxin

• AlphaFold DB: B1X2A9