Detailed information of TA system TA227797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1197700..1198229
• Replicon: chromosome
• Accession: NZ_CP089165
• Organism: Staphylococcus aureus strain UNC_SaCF07

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LTK18_RS05900
• Protein ID: WP_000621175.1
• Coordinates: 1197867..1198229 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LTK18_RS05895
• Protein ID: WP_000948331.1
• Coordinates: 1197700..1197870 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTK18_RS05865 (LTK18_05865)	1192737..1193297	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
LTK18_RS05870 (LTK18_05870)	1193505..1193984	+	480	WP_001287087.1	hypothetical protein	-
LTK18_RS05875 (LTK18_05875)	1193977..1195560	+	1584	WP_001294626.1	PH domain-containing protein	-
LTK18_RS05880 (LTK18_05880)	1195547..1196038	+	492	WP_001205912.1	PH domain-containing protein	-
LTK18_RS05885 (LTK18_05885)	1196042..1196401	+	360	WP_000581197.1	holo-ACP synthase	-
LTK18_RS05890 (LTK18_05890)	1196467..1197615	+	1149	WP_001281154.1	alanine racemase	-
LTK18_RS05895 (LTK18_05895)	1197700..1197870	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LTK18_RS05900 (LTK18_05900)	1197867..1198229	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LTK18_RS05905 (LTK18_05905)	1198579..1199580	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
LTK18_RS05910 (LTK18_05910)	1199699..1200025	+	327	WP_001052491.1	anti-sigma factor antagonist	-
LTK18_RS05915 (LTK18_05915)	1200027..1200506	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
LTK18_RS05920 (LTK18_05920)	1200481..1201251	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T227797 WP_000621175.1 NZ_CP089165:1197867-1198229 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T227797 NZ_CP123041:37091-37396 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT227797 WP_000948331.1 NZ_CP089165:1197700-1197870 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT227797 NZ_CP123041:36871-37089 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1