Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 33092..33606 Replicon chromosome
Accession NZ_CP089161
Organism Staphylococcus aureus strain UNC_SaCF13

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag LTK21_RS00155 Protein ID WP_000581792.1
Coordinates 33092..33301 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag LTK21_RS00160 Protein ID WP_000138932.1
Coordinates 33313..33606 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LTK21_RS00135 (LTK21_00135) 28600..29958 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
LTK21_RS00140 (LTK21_00140) 29973..31043 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
LTK21_RS00145 (LTK21_00145) 31361..32563 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
LTK21_RS00150 (LTK21_00150) 32827..32964 - 138 WP_000828354.1 Lmo0850 family protein -
LTK21_RS00155 (LTK21_00155) 33092..33301 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
LTK21_RS00160 (LTK21_00160) 33313..33606 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
LTK21_RS00165 (LTK21_00165) 33772..35256 + 1485 WP_000571549.1 cardiolipin synthase -
LTK21_RS00170 (LTK21_00170) 35284..35931 + 648 WP_191682978.1 HD domain-containing protein -
LTK21_RS00175 (LTK21_00175) 36944..37816 - 873 WP_000725802.1 membrane protein insertase YidC -
LTK21_RS00180 (LTK21_00180) 37902..38543 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T227760 WP_000581792.1 NZ_CP089161:c33301-33092 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T227760 NZ_CP123031:c63022-62717 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTCTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT227760 WP_000138932.1 NZ_CP089161:c33606-33313 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT227760 NZ_CP123031:c63242-63024 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References