Detailed information of TA system TA227758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2909642..2910171
• Replicon: chromosome
• Accession: NZ_CP089159
• Organism: Staphylococcus aureus strain UNC_SaCF18

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LTK20_RS14755
• Protein ID: WP_000621175.1
• Coordinates: 2909809..2910171 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LTK20_RS14750
• Protein ID: WP_000948331.1
• Coordinates: 2909642..2909812 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTK20_RS14720 (2904679)	2904679..2905239	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
LTK20_RS14725 (2905447)	2905447..2905926	+	480	WP_001287088.1	hypothetical protein	-
LTK20_RS14730 (2905919)	2905919..2907502	+	1584	WP_001294626.1	PH domain-containing protein	-
LTK20_RS14735 (2907489)	2907489..2907980	+	492	WP_001205910.1	PH domain-containing protein	-
LTK20_RS14740 (2907984)	2907984..2908343	+	360	WP_000581200.1	holo-ACP synthase	-
LTK20_RS14745 (2908409)	2908409..2909557	+	1149	WP_001281145.1	alanine racemase	-
LTK20_RS14750 (2909642)	2909642..2909812	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LTK20_RS14755 (2909809)	2909809..2910171	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LTK20_RS14760 (2910521)	2910521..2911522	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
LTK20_RS14765 (2911641)	2911641..2911967	+	327	WP_001052491.1	anti-sigma factor antagonist	-
LTK20_RS14770 (2911969)	2911969..2912448	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
LTK20_RS14775 (2912423)	2912423..2913193	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T227758 WP_000621175.1 NZ_CP089159:2909809-2910171 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T227758 NZ_CP123030:65905-66285 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCTGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT227758 WP_000948331.1 NZ_CP089159:2909642-2909812 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT227758 NZ_CP123030:65684-65905 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1