Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2889901..2890415 Replicon chromosome
Accession NZ_CP089159
Organism Staphylococcus aureus strain UNC_SaCF18

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag LTK20_RS14665 Protein ID WP_000581792.1
Coordinates 2890206..2890415 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag LTK20_RS14660 Protein ID WP_000138932.1
Coordinates 2889901..2890194 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LTK20_RS14640 (2885164) 2885164..2885805 + 642 WP_000483153.1 thiamine phosphate synthase -
LTK20_RS14645 (2885891) 2885891..2886763 + 873 WP_000725802.1 membrane protein insertase YidC -
LTK20_RS14650 (2887576) 2887576..2888223 - 648 WP_001187629.1 HD domain-containing protein -
LTK20_RS14655 (2888251) 2888251..2889735 - 1485 WP_000571549.1 cardiolipin synthase -
LTK20_RS14660 (2889901) 2889901..2890194 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
LTK20_RS14665 (2890206) 2890206..2890415 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
LTK20_RS14670 (2890543) 2890543..2890680 + 138 WP_000828354.1 Lmo0850 family protein -
LTK20_RS14675 (2890944) 2890944..2892146 - 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
LTK20_RS14680 (2892464) 2892464..2893534 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
LTK20_RS14685 (2893549) 2893549..2894907 + 1359 WP_000611462.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T227757 WP_000581792.1 NZ_CP089159:2890206-2890415 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T227757 NZ_CP123029:c4282041-4281868 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT227757 WP_000138932.1 NZ_CP089159:2889901-2890194 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT227757 NZ_CP123029:c4282365-4282069 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References