Detailed information of TA system TA227748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1339163..1339640
• Replicon: chromosome
• Accession: NZ_CP089159
• Organism: Staphylococcus aureus strain UNC_SaCF18

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: Q2G005
• Locus tag: LTK20_RS07035
• Protein ID: WP_000052078.1
• Coordinates: 1339452..1339640 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: LTK20_RS07030
• Protein ID: WP_001059690.1
• Coordinates: 1339163..1339423 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTK20_RS06995 (1334556)	1334556..1335272	-	717	WP_000150017.1	type I 3-dehydroquinate dehydratase	-
LTK20_RS07000 (1335420)	1335420..1335842	+	423	WP_000974460.1	organic hydroperoxide resistance protein	-
LTK20_RS07005 (1336241)	1336241..1336735	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
LTK20_RS07010 (1336890)	1336890..1337507	+	618	WP_000255561.1	LysE/ArgO family amino acid transporter	-
LTK20_RS07015 (1337580)	1337580..1338161	-	582	WP_001140686.1	histidine phosphatase family protein	-
LTK20_RS07020 (1338386)	1338386..1338622	+	237	WP_000368687.1	hypothetical protein	-
LTK20_RS07025 (1338619)	1338619..1338822	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
LTK20_RS07030 (1339163)	1339163..1339423	+	261	WP_001059690.1	hypothetical protein	Antitoxin
LTK20_RS07035 (1339452)	1339452..1339640	+	189	WP_000052078.1	hypothetical protein	Toxin
LTK20_RS07040 (1339828)	1339828..1340397	+	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
LTK20_RS07045 (1340485)	1340485..1340769	+	285	WP_001004395.1	hypothetical protein	-
LTK20_RS07050 (1340831)	1340831..1341049	+	219	WP_000005886.1	hypothetical protein	-
LTK20_RS07055 (1341545)	1341545..1341745	-	201	WP_001059082.1	cold-shock protein	-
LTK20_RS07060 (1342102)	1342102..1342788	-	687	WP_001548082.1	thermonuclease family protein	-
LTK20_RS07065 (1343129)	1343129..1343599	-	471	WP_000824472.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	selq / selk	1313874..1344956	31082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7172.84 Da
• Isoelectric Point: 9.9971

>T227748 WP_000052078.1 NZ_CP089159:1339452-1339640 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T227748 NZ_CP123029:1813752-1814006 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT227748 WP_001059690.1 NZ_CP089159:1339163-1339423 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT227748 NZ_CP123029:1813504-1813755 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0D6HZ40

Antitoxin

• AlphaFold DB: A0A0E0VN56