Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 235237..236013 Replicon chromosome
Accession NZ_CP089159
Organism Staphylococcus aureus strain UNC_SaCF18

Toxin (Protein)


Gene name tsbT Uniprot ID X5E2E6
Locus tag LTK20_RS01585 Protein ID WP_000031108.1
Coordinates 235861..236013 (+) Length 51 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID W8U4V4
Locus tag LTK20_RS01580 Protein ID WP_001251224.1
Coordinates 235237..235836 (+) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LTK20_RS01560 (231153) 231153..232610 + 1458 WP_000649907.1 ABC transporter permease subunit -
LTK20_RS01565 (232603) 232603..233325 + 723 WP_000590809.1 amino acid ABC transporter ATP-binding protein -
LTK20_RS01570 (233476) 233476..234603 + 1128 WP_000379978.1 tRNA epoxyqueuosine(34) reductase QueG -
LTK20_RS01575 (234608) 234608..235078 + 471 WP_000181398.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
LTK20_RS01580 (235237) 235237..235836 + 600 WP_001251224.1 glucosamine-6-phosphate isomerase Antitoxin
LTK20_RS01585 (235861) 235861..236013 + 153 WP_000031108.1 SAS053 family protein Toxin
LTK20_RS01590 (236790) 236790..237185 + 396 WP_000901021.1 hypothetical protein -
LTK20_RS01595 (237381) 237381..238766 + 1386 WP_000116224.1 class II fumarate hydratase -
LTK20_RS01600 (239229) 239229..240050 - 822 WP_000669376.1 RluA family pseudouridine synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(124-196)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5936.28 Da        Isoelectric Point: 3.8962

>T227742 WP_000031108.1 NZ_CP089159:235861-236013 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Download         Length: 153 bp

>T227742 NZ_CP123029:743097-743393 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22343.47 Da        Isoelectric Point: 5.1445

>AT227742 WP_001251224.1 NZ_CP089159:235237-235836 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Download         Length: 600 bp

>AT227742 NZ_CP123029:743395-743790 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X5E2E6

Antitoxin

Source ID Structure
AlphaFold DB W8U4V4

References