227732

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 776325..776854 Replicon chromosome
Accession NZ_CP089157
Organism Staphylococcus aureus strain UNC_SaCF30

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag LTK17_RS03865 Protein ID WP_000621176.1
Coordinates 776325..776687 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag LTK17_RS03870 Protein ID WP_000948331.1
Coordinates 776684..776854 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LTK17_RS03845 (LTK17_03845) 773304..774074 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
LTK17_RS03850 (LTK17_03850) 774049..774528 - 480 WP_001190825.1 anti-sigma B factor RsbW -
LTK17_RS03855 (LTK17_03855) 774530..774856 - 327 WP_001052491.1 anti-sigma factor antagonist -
LTK17_RS03860 (LTK17_03860) 774975..775976 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
LTK17_RS03865 (LTK17_03865) 776325..776687 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LTK17_RS03870 (LTK17_03870) 776684..776854 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LTK17_RS03875 (LTK17_03875) 776939..778087 - 1149 WP_001281140.1 alanine racemase -
LTK17_RS03880 (LTK17_03880) 778153..778512 - 360 WP_000581197.1 holo-ACP synthase -
LTK17_RS03885 (LTK17_03885) 778516..779007 - 492 WP_001286801.1 PH domain-containing protein -
LTK17_RS03890 (LTK17_03890) 779000..780577 - 1578 WP_001294646.1 PH domain-containing protein -
LTK17_RS03895 (LTK17_03895) 780570..781049 - 480 WP_001287082.1 hypothetical protein -
LTK17_RS03900 (LTK17_03900) 781258..781818 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T227732 WP_000621176.1 NZ_CP089157:c776687-776325 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T227732 NZ_CP123024:2909622-2910278 [Escherichia coli]
ATGGCTTATGATTACAGCGCCAGCCTCAACCCGCAAAAAGCTCTGATCTGGCGTATCGTCCATCGGGACAATATCCCGTG
GATTCTGGATAACGGGCTGCACTGCGGCAACAGTCTGGTGCAGGCGGAAAACTGGATCAATATCGGCAACCCGGAGCTGA
TCGGCAAGCGTGCCGGGCATCCGGTACCTGTCGGAACGGGCGGGACACTGCATGACTATGTGCCGTTTTATTTTACCCCC
TTCTCGCCAATGCTGATGAACATCCACAGCGGGCGCGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGCAACTTGCGAAACGTGGCAGCACACGACGTACCCTTCGTGTTTACCGACAGCCATGCTTACTATAACTGGACCAACT
ACTACACCAGTCTGGATAGTCTGGACCAGATCGACTGGCCCATTTTGCAGGCACGGGACTTTCGCCGCGATCCAGACGAT
CCGGCTAAATTTGAACGCTATCAGGCAGAGGCGCTAATCTGGCAACACTGCCCGATCTCTCTGCTGGATGGGATAATTTG
TTACAGCGAAGAGGTCAGGTTACAATTAGAACAATGGCTGTTTCAGCGTAATCTAACAATGTCCGTTCACACACGGGCTG
GATGGTACTTCTCATGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT227732 WP_000948331.1 NZ_CP089157:c776854-776684 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT227732 NZ_CP123024:2910275-2911342 [Escherichia coli]
ATGATCACATATACACAAGGCAATTTACTGGACGCACCGGTGGAAGCTCTGGTAAATACGGTTAATACCGTCGGTGTCAT
GGGGAAAGGCATCGCGTTGATGTTTAAAGAGCGCTTCCCCGAAAATATGAAAGTCTATGCCCTCGCCTGCAAACAAAAGC
AGGTGATCACCGGCAAAATGTTCATTACCGAAACCGGGGAACTGATGGGACCGCGCTGGATTGTTAATTTTCCGACCAAA
CAGCACTGGCGCGCTGATTCCCGCATGGAGTGGATTGAAGATGGCTTGCAGGACTTACGCCGCTTTTTGATTGAAGAGAA
CGTGCAGTCCATCGCCATTCCACCACTGGGCGCAGGCAACGGTGGTTTGAACTGGCCGGACGTTCGGGCGCAGATTGAAT
CTGCCCTTGGCGACCTTCAGGACGTTGATATCCTGATTTACCAACCGACTGAAAAATACCAGAACGTTGCCAAAAGCACT
GGCGTAAAAAAGCTCACTCCCGCCAGAGCCGCCATCGCTGAACTGGTGCGTCGCTACTGGGTATTGGGGATGGAGTGCAG
CCTACTGGAAATCCAGAAACTGGCCTGGCTGTTACAACGCGCCATTGAGCAGCATCAACAGGACGATATCCTCAAACTGC
GGTTTGAAGCTCACTATTATGGCCCCTATGCGCCCAATCTGAACCATCTGCTTAATGCGCTGGACGGCACCTATCTTAAA
GCCGAGAAACGCATCCCGGACAGCCAACCGCTGGATGTGATCTGGTTTAACGATCAGAAAAAAGAACACGTTAATGCGTA
TCTGAACAACGAAGCCAGAGAGTGGCTACCTGCGCTGGAGCAGGTCAGCCAGTTAATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGTTGCTGGCAACCGTAGACTGGTTGCTGAGCCGTGGCGAATGTCAACCAACGCTGGATTCCGTTAAGGAAGGG
CTGCACCAGTGGCCTGCGGGCGAACGCTGGGCCAGCCGTAAGTTGAGGCTGTTTGATAATAATAATCTGCAATTTGCCAT
TAACCGCGTAATGGAGTTCCATTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References