Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 664512..665041 Replicon chromosome
Accession NZ_CP089156
Organism Staphylococcus aureus strain UNC_SaCF35

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag LTK19_RS03710 Protein ID WP_000621176.1
Coordinates 664512..664874 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag LTK19_RS03715 Protein ID WP_000948331.1
Coordinates 664871..665041 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LTK19_RS03690 (LTK19_03690) 661491..662261 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
LTK19_RS03695 (LTK19_03695) 662236..662715 - 480 WP_001190825.1 anti-sigma B factor RsbW -
LTK19_RS03700 (LTK19_03700) 662717..663043 - 327 WP_001052491.1 anti-sigma factor antagonist -
LTK19_RS03705 (LTK19_03705) 663162..664163 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
LTK19_RS03710 (LTK19_03710) 664512..664874 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LTK19_RS03715 (LTK19_03715) 664871..665041 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LTK19_RS03720 (LTK19_03720) 665126..666274 - 1149 WP_001281140.1 alanine racemase -
LTK19_RS03725 (LTK19_03725) 666340..666699 - 360 WP_000581197.1 holo-ACP synthase -
LTK19_RS03730 (LTK19_03730) 666703..667194 - 492 WP_001286801.1 PH domain-containing protein -
LTK19_RS03735 (LTK19_03735) 667187..668764 - 1578 WP_001294646.1 PH domain-containing protein -
LTK19_RS03740 (LTK19_03740) 668757..669236 - 480 WP_001287082.1 hypothetical protein -
LTK19_RS03745 (LTK19_03745) 669445..670005 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T227722 WP_000621176.1 NZ_CP089156:c664874-664512 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T227722 NZ_CP123024:1111669-1111980 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT227722 WP_000948331.1 NZ_CP089156:c665041-664871 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT227722 NZ_CP123024:1111977-1112396 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References