Detailed information of TA system TA227710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 341260..342036
• Replicon: chromosome
• Accession: NZ_CP089154
• Organism: Staphylococcus aureus strain UNC_SaCF36

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: LTK15_RS02065
• Protein ID: WP_000031109.1
• Coordinates: 341884..342036 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: LTK15_RS02060
• Protein ID: WP_001251224.1
• Coordinates: 341260..341859 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LTK15_RS02040 (337176)	337176..338633	+	1458	WP_000649917.1	ABC transporter permease subunit	-
LTK15_RS02045 (338626)	338626..339348	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
LTK15_RS02050 (339499)	339499..340626	+	1128	WP_000379990.1	tRNA epoxyqueuosine(34) reductase QueG	-
LTK15_RS02055 (340631)	340631..341101	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
LTK15_RS02060 (341260)	341260..341859	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
LTK15_RS02065 (341884)	341884..342036	+	153	WP_000031109.1	SAS053 family protein	Toxin
LTK15_RS02070 (342804)	342804..343199	+	396	WP_000901019.1	hypothetical protein	-
LTK15_RS02075 (343395)	343395..344780	+	1386	WP_000116239.1	class II fumarate hydratase	-
LTK15_RS02080 (345237)	345237..346058	-	822	WP_000669377.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5950.31 Da
• Isoelectric Point: 3.9075

>T227710 WP_000031109.1 NZ_CP089154:341884-342036 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEESQ

Nucleotide

• Length: 153 bp

>T227710 NZ_CP123017:c4336988-4336815 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT227710 WP_001251224.1 NZ_CP089154:341260-341859 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT227710 NZ_CP123017:c4337312-4337016 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: W8U4V4