Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3523120..3523738 Replicon chromosome
Accession NZ_CP089142
Organism Escherichia coli strain XD35

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K0768_RS20495 Protein ID WP_001291435.1
Coordinates 3523520..3523738 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K0768_RS20490 Protein ID WP_000344800.1
Coordinates 3523120..3523494 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0768_RS20480 (3518209) 3518209..3519402 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K0768_RS20485 (3519425) 3519425..3522574 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
K0768_RS20490 (3523120) 3523120..3523494 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K0768_RS20495 (3523520) 3523520..3523738 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K0768_RS20500 (3523910) 3523910..3524461 + 552 WP_000102564.1 maltose O-acetyltransferase -
K0768_RS20505 (3524577) 3524577..3525047 + 471 WP_000136192.1 YlaC family protein -
K0768_RS20510 (3525211) 3525211..3526761 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K0768_RS20515 (3526803) 3526803..3527156 - 354 WP_000878140.1 DUF1428 domain-containing protein -
K0768_RS20525 (3527535) 3527535..3527846 + 312 WP_000409911.1 MGMT family protein -
K0768_RS20530 (3527877) 3527877..3528449 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227688 WP_001291435.1 NZ_CP089142:3523520-3523738 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T227688 NZ_CP123014:c86736-86431 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTCTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT227688 WP_000344800.1 NZ_CP089142:3523120-3523494 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT227688 NZ_CP123014:c86956-86738 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References