Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 56976..57577 Replicon plasmid pXD35003
Accession NZ_CP089136
Organism Escherichia coli strain XD35

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag K0768_RS02370 Protein ID WP_001216034.1
Coordinates 56976..57356 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag K0768_RS02375 Protein ID WP_001190712.1
Coordinates 57356..57577 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0768_RS02345 (LS330_02345) 52417..53901 - 1485 WP_072643598.1 terminase -
K0768_RS02350 (LS330_02350) 53901..55094 - 1194 WP_054486466.1 hypothetical protein -
K0768_RS02355 (LS330_02355) 55180..55632 - 453 WP_001326849.1 late promoter-activating protein -
K0768_RS02360 (LS330_02360) 55721..56764 - 1044 WP_072643599.1 DUF968 domain-containing protein -
K0768_RS02365 (LS330_02365) 56792..56971 - 180 WP_000113018.1 hypothetical protein -
K0768_RS02370 (LS330_02370) 56976..57356 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K0768_RS02375 (LS330_02375) 57356..57577 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K0768_RS02380 (LS330_02380) 57760..59316 + 1557 WP_072643605.1 type I restriction-modification system subunit M -
K0768_RS02385 (LS330_02385) 59313..59654 + 342 WP_153520317.1 restriction endonuclease subunit S -
K0768_RS02390 (LS330_02390) 59765..60745 - 981 WP_000082736.1 IS5-like element ISEc68 family transposase -
K0768_RS02395 (LS330_02395) 60865..61731 + 867 WP_232044467.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid oqxB / oqxA / sul1 / blaTEM-1B / rmtB / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE - 1..91079 91079
- flank IS/Tn - - 59765..60745 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T227664 WP_001216034.1 NZ_CP089136:c57356-56976 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T227664 NZ_CP123009:4101545-4101850 [Escherichia coli O155]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCCCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT227664 WP_001190712.1 NZ_CP089136:c57577-57356 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT227664 NZ_CP123009:4101325-4101543 [Escherichia coli O155]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References