Detailed information of TA system TA227663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 114428..115071
• Replicon: plasmid pXD35002
• Accession: NZ_CP089135
• Organism: Escherichia coli strain XD35

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K0768_RS01970
• Protein ID: WP_016236302.1
• Coordinates: 114655..115071 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: K0768_RS01965
• Protein ID: WP_001261282.1
• Coordinates: 114428..114658 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0768_RS01945 (110977)	110977..111732	-	756	WP_000852146.1	replication initiation protein RepE	-
K0768_RS01950 (112506)	112506..113312	-	807	WP_000016982.1	site-specific integrase	-
K0768_RS01955 (113313)	113313..113618	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	-
K0768_RS01960 (113620)	113620..113838	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-
K0768_RS01965 (114428)	114428..114658	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K0768_RS01970 (114655)	114655..115071	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
K0768_RS01975 (115145)	115145..116707	+	1563	WP_004206609.1	AAA family ATPase	-
K0768_RS01980 (116692)	116692..117714	+	1023	WP_000361404.1	helicase UvrD	-
K0768_RS01985 (117895)	117895..118125	-	231	Protein_136	IS1 family transposase	-
K0768_RS01990 (118180)	118180..118884	-	705	WP_009364894.1	IS6-like element IS26 family transposase	-
K0768_RS01995 (119083)	119083..119466	+	384	WP_001514447.1	transposase	-
K0768_RS02000 (119463)	119463..119810	+	348	WP_000609174.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / tet(A) / aac(3)-IVa / aph(4)-Ia / floR / mph(A) / erm(B) / sul3	-	1..129210	129210

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T227663 WP_016236302.1 NZ_CP089135:114655-115071 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T227663 NZ_CP123009:c3952070-3951759 [Escherichia coli O155]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT227663 WP_001261282.1 NZ_CP089135:114428-114658 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT227663 NZ_CP123009:c3951758-3951468 [Escherichia coli O155]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3