Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 8066..9689 Replicon plasmid pXD35001
Accession NZ_CP089134
Organism Escherichia coli strain XD35

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag K0768_RS00060 Protein ID WP_000088046.1
Coordinates 8066..9391 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag K0768_RS00065 Protein ID WP_024136327.1
Coordinates 9396..9689 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0768_RS00025 (LS330_00025) 3546..4817 + 1272 WP_000592090.1 type-F conjugative transfer system secretin TraK -
K0768_RS00030 (LS330_00030) 4819..5280 + 462 WP_000521240.1 plasmid transfer protein HtdO -
K0768_RS00035 (LS330_00035) 5270..6235 + 966 WP_269903009.1 conjugal transfer protein TraB -
K0768_RS00040 (LS330_00040) 6272..6976 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
K0768_RS00045 (LS330_00045) 7029..7274 - 246 Protein_8 HNH endonuclease -
K0768_RS00050 (LS330_00050) 7287..7697 - 411 WP_153449092.1 hypothetical protein -
K0768_RS00055 (LS330_00055) 7799..8005 - 207 WP_000134171.1 hypothetical protein -
K0768_RS00060 (LS330_00060) 8066..9391 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
K0768_RS00065 (LS330_00065) 9396..9689 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
K0768_RS00070 (LS330_00070) 10293..10685 - 393 WP_000341066.1 hypothetical protein -
K0768_RS00075 (LS330_00075) 11058..11399 + 342 WP_001151575.1 hypothetical protein -
K0768_RS00080 (LS330_00080) 11491..11682 + 192 WP_000398480.1 hypothetical protein -
K0768_RS00085 (LS330_00085) 11732..12013 + 282 WP_000077926.1 hypothetical protein -
K0768_RS26845 12354..12485 - 132 WP_000374059.1 hypothetical protein -
K0768_RS00090 (LS330_00090) 12576..13817 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / ant(3'')-Ia / lnu(F) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aph(4)-Ia / aac(3)-IVa / sul3 / cmlA1 / aadA2 / tet(A) / qnrS1 / ARR-3 / blaOXA-10 / dfrA14 - 1..244138 244138


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T227657 WP_000088046.1 NZ_CP089134:c9391-8066 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T227657 NZ_CP123009:1962390-1962725 [Escherichia coli O155]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT227657 WP_024136327.1 NZ_CP089134:c9689-9396 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT227657 NZ_CP123009:1962142-1962390 [Escherichia coli O155]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References