Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 130166..130809 Replicon plasmid pEH13_2
Accession NZ_CP089099
Organism Klebsiella pneumoniae strain EH13

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag LSG42_RS28485 Protein ID WP_001034044.1
Coordinates 130393..130809 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag LSG42_RS28480 Protein ID WP_001261286.1
Coordinates 130166..130396 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSG42_RS28465 (125337) 125337..125567 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
LSG42_RS28470 (125564) 125564..125980 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
LSG42_RS28475 (126025) 126025..129819 - 3795 WP_001144732.1 hypothetical protein -
LSG42_RS28480 (130166) 130166..130396 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LSG42_RS28485 (130393) 130393..130809 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
LSG42_RS28490 (130884) 130884..132449 + 1566 WP_001128474.1 AAA family ATPase -
LSG42_RS28495 (132434) 132434..133456 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / erm(42) / sul2 / tet(M) / mph(A) / erm(B) / blaCTX-M-14 / qacE / cmlA1 / sitABCD iutA / iucD / iucC / iucB / iucA 1..152520 152520
- flank IS/Tn - - 133710..134213 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T227656 WP_001034044.1 NZ_CP089099:130393-130809 [Klebsiella pneumoniae]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T227656 NZ_CP123009:1826055-1826462 [Escherichia coli O155]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT227656 WP_001261286.1 NZ_CP089099:130166-130396 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT227656 NZ_CP123009:1825808-1826074 [Escherichia coli O155]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References