Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 125337..125980 Replicon plasmid pEH13_2
Accession NZ_CP089099
Organism Klebsiella pneumoniae strain EH13

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag LSG42_RS28470 Protein ID WP_001034046.1
Coordinates 125564..125980 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag LSG42_RS28465 Protein ID WP_001261278.1
Coordinates 125337..125567 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSG42_RS28435 (120847) 120847..121152 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
LSG42_RS28440 (121154) 121154..121372 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
LSG42_RS28445 (121940) 121940..122452 + 513 WP_000151784.1 hypothetical protein -
LSG42_RS28450 (122486) 122486..123619 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
LSG42_RS28455 (123786) 123786..124559 - 774 WP_000905949.1 hypothetical protein -
LSG42_RS28460 (124572) 124572..125072 - 501 WP_000528931.1 HEPN family nuclease -
LSG42_RS28465 (125337) 125337..125567 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LSG42_RS28470 (125564) 125564..125980 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
LSG42_RS28475 (126025) 126025..129819 - 3795 WP_001144732.1 hypothetical protein -
LSG42_RS28480 (130166) 130166..130396 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
LSG42_RS28485 (130393) 130393..130809 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / erm(42) / sul2 / tet(M) / mph(A) / erm(B) / blaCTX-M-14 / qacE / cmlA1 / sitABCD iutA / iucD / iucC / iucB / iucA 1..152520 152520


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T227655 WP_001034046.1 NZ_CP089099:125564-125980 [Klebsiella pneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T227655 NZ_CP123009:1603113-1603655 [Escherichia coli O155]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT227655 WP_001261278.1 NZ_CP089099:125337-125567 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT227655 NZ_CP123009:1602818-1603129 [Escherichia coli O155]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References