Detailed information of TA system TA227654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 120847..121372
• Replicon: plasmid pEH13_2
• Accession: NZ_CP089099
• Organism: Klebsiella pneumoniae strain EH13

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: LSG42_RS28435
• Protein ID: WP_001159868.1
• Coordinates: 120847..121152 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: LSG42_RS28440
• Protein ID: WP_000813634.1
• Coordinates: 121154..121372 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG42_RS28420 (116757)	116757..117923	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
LSG42_RS28425 (118511)	118511..119266	-	756	WP_000852146.1	replication initiation protein RepE	-
LSG42_RS28430 (120040)	120040..120846	-	807	WP_000016982.1	site-specific integrase	-
LSG42_RS28435 (120847)	120847..121152	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
LSG42_RS28440 (121154)	121154..121372	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LSG42_RS28445 (121940)	121940..122452	+	513	WP_000151784.1	hypothetical protein	-
LSG42_RS28450 (122486)	122486..123619	-	1134	WP_001542067.1	DUF3800 domain-containing protein	-
LSG42_RS28455 (123786)	123786..124559	-	774	WP_000905949.1	hypothetical protein	-
LSG42_RS28460 (124572)	124572..125072	-	501	WP_000528931.1	HEPN family nuclease	-
LSG42_RS28465 (125337)	125337..125567	+	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	-
LSG42_RS28470 (125564)	125564..125980	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / erm(42) / sul2 / tet(M) / mph(A) / erm(B) / blaCTX-M-14 / qacE / cmlA1 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..152520	152520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T227654 WP_001159868.1 NZ_CP089099:c121152-120847 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T227654 NZ_CP123009:1569141-1569359 [Escherichia coli O155]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT227654 WP_000813634.1 NZ_CP089099:c121372-121154 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT227654 NZ_CP123009:1568741-1569115 [Escherichia coli O155]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2