Detailed information of TA system TA227649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 247368..248278
• Replicon: plasmid pEH13_1
• Accession: NZ_CP089098
• Organism: Klebsiella pneumoniae strain EH13

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LSG42_RS27320
• Protein ID: WP_004026354.1
• Coordinates: 247808..248278 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: LSG42_RS27315
• Protein ID: WP_004181895.1
• Coordinates: 247368..247811 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG42_RS27295 (LSG42_27295)	242585..243292	+	708	WP_072198721.1	hypothetical protein	-
LSG42_RS27300 (LSG42_27300)	243297..244328	+	1032	WP_170856552.1	CRISPR-associated protein Csf2	-
LSG42_RS27305 (LSG42_27305)	245574..245996	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
LSG42_RS27310 (LSG42_27310)	245996..247267	+	1272	WP_073558158.1	translesion error-prone DNA polymerase V subunit UmuC	-
LSG42_RS27315 (LSG42_27315)	247368..247811	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
LSG42_RS27320 (LSG42_27320)	247808..248278	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LSG42_RS27325 (LSG42_27325)	248733..248921	-	189	WP_024198076.1	hypothetical protein	-
LSG42_RS27330 (LSG42_27330)	249534..249794	-	261	WP_032719456.1	hypothetical protein	-
LSG42_RS27335 (LSG42_27335)	250517..250675	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
LSG42_RS27340 (LSG42_27340)	251281..252255	+	975	WP_171003093.1	Hok/Gef family protein	-
LSG42_RS27345 (LSG42_27345)	252453..252830	-	378	WP_004181901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(D) / aph(6)-Id / aph(3'')-Ib / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB4 / blaDHA-1 / blaTEM-1B	-	1..330084	330084

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T227649 WP_004026354.1 NZ_CP089098:247808-248278 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T227649 NZ_CP123009:c948217-947819 [Escherichia coli O155]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT227649 WP_004181895.1 NZ_CP089098:247368-247811 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT227649 NZ_CP123009:c948444-948217 [Escherichia coli O155]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A2J4RLY9