Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5222801..5223426 Replicon chromosome
Accession NZ_CP089097
Organism Klebsiella pneumoniae strain EH13

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LSG42_RS25530 Protein ID WP_002882817.1
Coordinates 5222801..5223184 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LSG42_RS25535 Protein ID WP_004150355.1
Coordinates 5223184..5223426 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSG42_RS25515 (LSG42_25515) 5220167..5221069 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LSG42_RS25520 (LSG42_25520) 5221066..5221701 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LSG42_RS25525 (LSG42_25525) 5221698..5222627 + 930 WP_004146237.1 formate dehydrogenase accessory protein FdhE -
LSG42_RS25530 (LSG42_25530) 5222801..5223184 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LSG42_RS25535 (LSG42_25535) 5223184..5223426 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LSG42_RS25540 (LSG42_25540) 5223631..5224548 + 918 WP_032437446.1 alpha/beta hydrolase -
LSG42_RS25545 (LSG42_25545) 5224562..5225503 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
LSG42_RS25550 (LSG42_25550) 5225548..5225985 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LSG42_RS25555 (LSG42_25555) 5225982..5226842 - 861 WP_004146232.1 virulence factor BrkB family protein -
LSG42_RS25560 (LSG42_25560) 5226836..5227435 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T227647 WP_002882817.1 NZ_CP089097:c5223184-5222801 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T227647 NZ_CP123009:c476004-475750 [Escherichia coli O155]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT227647 WP_004150355.1 NZ_CP089097:c5223426-5223184 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT227647 NZ_CP123009:c476252-476001 [Escherichia coli O155]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References