Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4631728..4632538 Replicon chromosome
Accession NZ_CP089097
Organism Klebsiella pneumoniae strain EH13

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LSG42_RS22700 Protein ID WP_004178461.1
Coordinates 4631728..4632261 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LSG42_RS22705 Protein ID WP_002887278.1
Coordinates 4632272..4632538 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSG42_RS22695 (LSG42_22695) 4630559..4631680 + 1122 WP_002887282.1 cupin domain-containing protein -
LSG42_RS22700 (LSG42_22700) 4631728..4632261 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LSG42_RS22705 (LSG42_22705) 4632272..4632538 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LSG42_RS22710 (LSG42_22710) 4632641..4634074 - 1434 WP_049024943.1 Cu(+)/Ag(+) sensor histidine kinase -
LSG42_RS22715 (LSG42_22715) 4634064..4634747 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
LSG42_RS22720 (LSG42_22720) 4634919..4636304 + 1386 WP_049024944.1 efflux transporter outer membrane subunit -
LSG42_RS22725 (LSG42_22725) 4636322..4636666 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T227644 WP_004178461.1 NZ_CP089097:c4632261-4631728 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T227644 NZ_CP123008:4658541-4658819 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT227644 WP_002887278.1 NZ_CP089097:c4632538-4632272 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT227644 NZ_CP123008:4658278-4658538 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References