Detailed information of TA system TA227641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2690357..2691945
• Replicon: chromosome
• Accession: NZ_CP089097
• Organism: Klebsiella pneumoniae strain EH13

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LSG42_RS13325
• Protein ID: WP_032436337.1
• Coordinates: 2690357..2691679 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: LSG42_RS13330
• Protein ID: WP_002904790.1
• Coordinates: 2691679..2691945 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG42_RS13310 (LSG42_13310)	2685414..2687915	+	2502	WP_231822770.1	fimbria/pilus outer membrane usher protein	-
LSG42_RS13315 (LSG42_13315)	2687903..2688907	+	1005	WP_032436338.1	fimbrial protein	-
LSG42_RS13320 (LSG42_13320)	2689048..2690340	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LSG42_RS13325 (LSG42_13325)	2690357..2691679	-	1323	WP_032436337.1	type II toxin-antitoxin system HipA family toxin	Toxin
LSG42_RS13330 (LSG42_13330)	2691679..2691945	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LSG42_RS13335 (LSG42_13335)	2692332..2692658	-	327	WP_032436336.1	hydroxyisourate hydrolase	-
LSG42_RS13340 (LSG42_13340)	2692655..2693155	-	501	WP_032436335.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LSG42_RS13345 (LSG42_13345)	2693133..2694512	-	1380	WP_032412205.1	nucleobase:cation symporter-2 family protein	-
LSG42_RS13350 (LSG42_13350)	2694858..2696012	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LSG42_RS13355 (LSG42_13355)	2695993..2696916	-	924	WP_032436334.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49106.39 Da
• Isoelectric Point: 7.5617

>T227641 WP_032436337.1 NZ_CP089097:c2691679-2690357 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGDHWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T227641 NZ_CP123008:3910764-3911075 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT227641 WP_002904790.1 NZ_CP089097:c2691945-2691679 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT227641 NZ_CP123008:3911076-3911366 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8