Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 18703..19222 Replicon plasmid pK21EFM001
Accession NZ_CP089093
Organism Enterococcus faecium strain V13-21-E11-012-001

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag LRL10_RS13350 Protein ID WP_000588503.1
Coordinates 18965..19222 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag LRL10_RS13345 Protein ID WP_000388479.1
Coordinates 18703..18972 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRL10_RS13300 (LRL10_14900) 13815..14075 + 261 WP_002295623.1 hypothetical protein -
LRL10_RS13305 (LRL10_14905) 14512..14718 + 207 WP_002347218.1 hypothetical protein -
LRL10_RS13310 (LRL10_14910) 14718..14969 + 252 WP_002296238.1 hypothetical protein -
LRL10_RS13315 (LRL10_14915) 14984..15421 + 438 WP_002300851.1 hypothetical protein -
LRL10_RS13320 (LRL10_14920) 15414..16121 + 708 WP_002303117.1 hypothetical protein -
LRL10_RS13325 (LRL10_14925) 16286..16486 + 201 WP_002347143.1 hypothetical protein -
LRL10_RS13330 (LRL10_14930) 16502..16681 + 180 WP_001120991.1 hypothetical protein -
LRL10_RS13335 (LRL10_14935) 16886..17290 + 405 WP_002287522.1 IS200/IS605-like element ISEfa4 family transposase -
LRL10_RS13340 (LRL10_14940) 17307..18455 + 1149 WP_002287525.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
LRL10_RS13345 (LRL10_14945) 18703..18972 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LRL10_RS13350 (LRL10_14950) 18965..19222 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
LRL10_RS13355 (LRL10_14955) 19681..19836 + 156 WP_002347142.1 hypothetical protein -
LRL10_RS13360 (LRL10_14960) 19860..20684 + 825 WP_001809248.1 hypothetical protein -
LRL10_RS13365 (LRL10_14965) 20849..21463 - 615 WP_001261742.1 recombinase family protein -
LRL10_RS13370 (LRL10_14970) 21701..22123 + 423 WP_002303116.1 hypothetical protein -
LRL10_RS13375 (LRL10_14975) 22133..22336 + 204 WP_002299573.1 hypothetical protein -
LRL10_RS13380 (LRL10_14980) 22547..23131 + 585 WP_002299575.1 recombinase family protein -
LRL10_RS13385 (LRL10_14985) 23320..24000 - 681 WP_010861579.1 IS6-like element IS1216 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..201227 201227
- inside IScluster/Tn - - 16886..24000 7114


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T227620 WP_000588503.1 NZ_CP089093:18965-19222 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T227620 NZ_CP123006:71461-71757 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTACCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGTAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGTGTGGACGCCCAACGGCATTTGTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT227620 WP_000388479.1 NZ_CP089093:18703-18972 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT227620 NZ_CP123006:71223-71471 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CCTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References