Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 828485..829114 Replicon chromosome
Accession NZ_CP089090
Organism Listeria marthii strain UTK_C1-0015-E1

Toxin (Protein)


Gene name pemK Uniprot ID A0A6Z2PJ29
Locus tag LAX80_RS04040 Protein ID WP_031695189.1
Coordinates 828767..829114 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID H1GC21
Locus tag LAX80_RS04035 Protein ID WP_003761302.1
Coordinates 828485..828763 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAX80_RS04015 (LAX80_004010) 823789..825264 + 1476 WP_230911227.1 PH domain-containing protein -
LAX80_RS04020 (LAX80_004015) 825416..826795 + 1380 WP_230906381.1 protoporphyrinogen oxidase -
LAX80_RS04025 (LAX80_004020) 826797..827153 + 357 WP_008947275.1 holo-ACP synthase -
LAX80_RS04030 (LAX80_004025) 827172..828278 + 1107 WP_230911228.1 alanine racemase -
LAX80_RS04035 (LAX80_004030) 828485..828763 + 279 WP_003761302.1 CopG family ribbon-helix-helix protein Antitoxin
LAX80_RS04040 (LAX80_004035) 828767..829114 + 348 WP_031695189.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LAX80_RS04045 (LAX80_004040) 829366..830202 + 837 WP_003721456.1 STAS domain-containing protein -
LAX80_RS04050 (LAX80_004045) 830208..830564 + 357 WP_003721457.1 STAS domain-containing protein -
LAX80_RS04055 (LAX80_004050) 830567..830977 + 411 WP_003738506.1 anti-sigma regulatory factor -
LAX80_RS04060 (LAX80_004055) 830994..831998 + 1005 WP_230906379.1 PP2C family protein-serine/threonine phosphatase -
LAX80_RS04065 (LAX80_004060) 832147..832491 + 345 WP_003721460.1 anti sigma b factor antagonist RsbV -
LAX80_RS04070 (LAX80_004065) 832475..832948 + 474 WP_003721461.1 anti-sigma B factor RsbW -
LAX80_RS04075 (LAX80_004070) 832926..833705 + 780 WP_230911229.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12801.89 Da        Isoelectric Point: 7.2038

>T227618 WP_031695189.1 NZ_CP089090:828767-829114 [Listeria marthii]
MMVKRGDVYYADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAKIQKAKLPTHVEATRKDGFERDSVILLEQIR
TIDKQRLTDKITHLDEELMAKVNKALEVSLGVVEF

Download         Length: 348 bp

>T227618 NZ_CP123005:c1401068-1400775 [Sinorhizobium meliloti]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10605.13 Da        Isoelectric Point: 4.2980

>AT227618 WP_003761302.1 NZ_CP089090:828485-828763 [Listeria marthii]
VLEKEKRMIISVELTQEMVQELDVVVEKEKMGRSEVIMEATQQFLQEKRARELRDEMERGYAEMATINFAIACECTHVEA
EAEDRNISILGG

Download         Length: 279 bp

>AT227618 NZ_CP123005:c1401373-1401065 [Sinorhizobium meliloti]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6Z2PJ29

Antitoxin

Source ID Structure
AlphaFold DB H1GC21

References