227617

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 955592..956221 Replicon chromosome
Accession NZ_CP089089
Organism Listeria marthii strain UTK_C1-0024-E1

Toxin (Protein)


Gene name pemK Uniprot ID A0A6Z2PJ29
Locus tag LAX74_RS04730 Protein ID WP_031695189.1
Coordinates 955874..956221 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID H1GC21
Locus tag LAX74_RS04725 Protein ID WP_003761302.1
Coordinates 955592..955870 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAX74_RS04705 (LAX74_004705) 950896..952371 + 1476 WP_230906382.1 PH domain-containing protein -
LAX74_RS04710 (LAX74_004710) 952523..953902 + 1380 WP_230906381.1 protoporphyrinogen oxidase -
LAX74_RS04715 (LAX74_004715) 953904..954260 + 357 WP_008947275.1 holo-ACP synthase -
LAX74_RS04720 (LAX74_004720) 954279..955385 + 1107 WP_230906380.1 alanine racemase -
LAX74_RS04725 (LAX74_004725) 955592..955870 + 279 WP_003761302.1 CopG family ribbon-helix-helix protein Antitoxin
LAX74_RS04730 (LAX74_004730) 955874..956221 + 348 WP_031695189.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LAX74_RS04735 (LAX74_004735) 956472..957308 + 837 WP_003721456.1 STAS domain-containing protein -
LAX74_RS04740 (LAX74_004740) 957314..957670 + 357 WP_003721457.1 STAS domain-containing protein -
LAX74_RS04745 (LAX74_004745) 957673..958083 + 411 WP_003738506.1 anti-sigma regulatory factor -
LAX74_RS04750 (LAX74_004750) 958100..959104 + 1005 WP_230906379.1 PP2C family protein-serine/threonine phosphatase -
LAX74_RS04755 (LAX74_004755) 959253..959597 + 345 WP_003721460.1 anti sigma b factor antagonist RsbV -
LAX74_RS04760 (LAX74_004760) 959581..960054 + 474 WP_003721461.1 anti-sigma B factor RsbW -
LAX74_RS04765 (LAX74_004765) 960032..960811 + 780 WP_003721462.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12801.89 Da        Isoelectric Point: 7.2038

>T227617 WP_031695189.1 NZ_CP089089:955874-956221 [Listeria marthii]
MMVKRGDVYYADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAKIQKAKLPTHVEATRKDGFERDSVILLEQIR
TIDKQRLTDKITHLDEELMAKVNKALEVSLGVVEF

Download         Length: 348 bp

>T227617 NZ_CP123005:c1362445-1362152 [Sinorhizobium meliloti]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10605.13 Da        Isoelectric Point: 4.2980

>AT227617 WP_003761302.1 NZ_CP089089:955592-955870 [Listeria marthii]
VLEKEKRMIISVELTQEMVQELDVVVEKEKMGRSEVIMEATQQFLQEKRARELRDEMERGYAEMATINFAIACECTHVEA
EAEDRNISILGG

Download         Length: 279 bp

>AT227617 NZ_CP123005:c1362750-1362442 [Sinorhizobium meliloti]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6Z2PJ29

Antitoxin

Source ID Structure
AlphaFold DB H1GC21

References