Detailed information of TA system TA227616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 5968580..5969838
• Replicon: chromosome
• Accession: NZ_CP089068
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF13

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LSP21_RS27350
• Protein ID: WP_003163133.1
• Coordinates: 5968580..5969335 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: LSP21_RS27355
• Protein ID: WP_003086329.1
• Coordinates: 5969470..5969838 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP21_RS27340 (LSP21_27350)	5963752..5966295	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
LSP21_RS27345 (LSP21_27355)	5966450..5967811	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
LSP21_RS27350 (LSP21_27360)	5968580..5969335	-	756	WP_003163133.1	RES family NAD+ phosphorylase	Toxin
LSP21_RS27355 (LSP21_27365)	5969470..5969838	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
LSP21_RS27360 (LSP21_27370)	5969911..5971197	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
LSP21_RS27365 (LSP21_27375)	5971253..5972746	-	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
LSP21_RS27370 (LSP21_27380)	5972921..5973397	+	477	WP_003112531.1	hypothetical protein	-
LSP21_RS27375 (LSP21_27385)	5973400..5973675	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T227616 WP_003163133.1 NZ_CP089068:c5969335-5968580 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T227616 NZ_CP123005:1157228-1157482 [Sinorhizobium meliloti]
ATGAACAGCAAGCAGAGAAAGACCCTCGCGGCCGTATTCAAGGACCCGGCCTCGGGAACGATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCGGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGCATCATTG
CGAGCTTTCATCGACCTCATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT227616 WP_003086329.1 NZ_CP089068:c5969838-5969470 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT227616 NZ_CP123005:1157479-1157835 [Sinorhizobium meliloti]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGAAGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCGGTCGATGACT
ACCTTGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTCAACCAGTGGGCAGAAGAGGTGCTGGAAGAAGC
CGCCGGCCATTTTGCCGAGGCGCGCCTGTCGGCGTAG

Structures

Antitoxin

• AlphaFold DB: V6AJU7