Detailed information of TA system TA227612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1665176..1665681
• Replicon: chromosome
• Accession: NZ_CP089068
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF13

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: LSP21_RS07620
• Protein ID: WP_003083773.1
• Coordinates: 1665176..1665457 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: LSP21_RS07625
• Protein ID: WP_003083775.1
• Coordinates: 1665454..1665681 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP21_RS07595 (LSP21_07605)	1660427..1661776	+	1350	WP_003119513.1	C4-dicarboxylate transporter DctA	-
LSP21_RS07600 (LSP21_07610)	1661825..1662511	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
LSP21_RS07605 (LSP21_07615)	1662612..1663346	+	735	WP_004346926.1	GntR family transcriptional regulator	-
LSP21_RS07610 (LSP21_07620)	1663526..1663936	+	411	WP_003101225.1	aegerolysin family protein	-
LSP21_RS07615 (LSP21_07625)	1663968..1664876	-	909	WP_003083769.1	LysR family transcriptional regulator	-
LSP21_RS07620 (LSP21_07630)	1665176..1665457	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
LSP21_RS07625 (LSP21_07635)	1665454..1665681	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
LSP21_RS07630 (LSP21_07640)	1665857..1666477	-	621	WP_003101226.1	hypothetical protein	-
LSP21_RS07635 (LSP21_07645)	1666578..1667078	+	501	WP_003112629.1	LEA type 2 family protein	-
LSP21_RS07640 (LSP21_07650)	1667151..1667492	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
LSP21_RS07645 (LSP21_07655)	1667574..1669001	-	1428	WP_003083784.1	GABA permease	-
LSP21_RS07650 (LSP21_07660)	1669170..1670663	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T227612 WP_003083773.1 NZ_CP089068:c1665457-1665176 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T227612 NZ_CP123003:c3743525-3743121 [Sinorhizobium meliloti]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT227612 WP_003083775.1 NZ_CP089068:c1665681-1665454 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT227612 NZ_CP123003:c3743782-3743522 [Sinorhizobium meliloti]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9