Detailed information of TA system TA227606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 3077859..3079117
• Replicon: chromosome
• Accession: NZ_CP089067
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF19

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LSP16_RS14755
• Protein ID: WP_003449003.1
• Coordinates: 3078362..3079117 (+)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: LSP16_RS14750
• Protein ID: WP_003086329.1
• Coordinates: 3077859..3078227 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP16_RS14730 (LSP16_14720)	3074019..3074294	+	276	WP_010792073.1	zinc ribbon domain-containing protein	-
LSP16_RS14735 (LSP16_14725)	3074297..3074773	-	477	WP_003140638.1	hypothetical protein	-
LSP16_RS14740 (LSP16_14730)	3074948..3076444	+	1497	WP_057379880.1	aldehyde dehydrogenase family protein	-
LSP16_RS14745 (LSP16_14735)	3076500..3077786	+	1287	WP_219832977.1	FAD-binding oxidoreductase	-
LSP16_RS14750 (LSP16_14740)	3077859..3078227	+	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
LSP16_RS14755 (LSP16_14745)	3078362..3079117	+	756	WP_003449003.1	RES family NAD+ phosphorylase	Toxin
LSP16_RS14760 (LSP16_14750)	3079888..3081249	+	1362	WP_003081943.1	DNA recombination protein RmuC	-
LSP16_RS14765 (LSP16_14755)	3081404..3083947	-	2544	WP_003123188.1	acyl-homoserine-lactone acylase QuiP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28292.45 Da
• Isoelectric Point: 7.7294

>T227606 WP_003449003.1 NZ_CP089067:3078362-3079117 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T227606 NZ_CP123003:c1188573-1188277 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT227606 WP_003086329.1 NZ_CP089067:3077859-3078227 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT227606 NZ_CP123003:c1188263-1187955 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7