Detailed information of TA system TA022760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 654839..655480
• Replicon: chromosome
• Accession: NC_010515
• Organism: Burkholderia cenocepacia MC0-3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B1K4X0
• Locus tag: BCENMC03_RS18925
• Protein ID: WP_006479524.1
• Coordinates: 654839..655177 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BCENMC03_RS18930
• Protein ID: WP_006490972.1
• Coordinates: 655181..655480 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCENMC03_RS18915	651357..652790	-	1434	WP_012338512.1	aldehyde dehydrogenase family protein	-
BCENMC03_RS18920	652819..654459	-	1641	WP_011548230.1	acetolactate synthase large subunit	-
BCENMC03_RS18925	654839..655177	+	339	WP_006479524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BCENMC03_RS18930	655181..655480	+	300	WP_006490972.1	putative addiction module antidote protein	Antitoxin
BCENMC03_RS18935	655529..656815	-	1287	WP_012338513.1	DUF445 domain-containing protein	-
BCENMC03_RS18940	656967..658187	-	1221	WP_012338514.1	FAD-dependent oxidoreductase	-
BCENMC03_RS18945	658177..658503	-	327	WP_006479527.1	non-heme iron oxygenase ferredoxin subunit	-
BCENMC03_RS18950	658539..659015	-	477	WP_006490977.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
BCENMC03_RS18955	659030..660301	-	1272	WP_012338515.1	Rieske 2Fe-2S domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (26-98)

Antitoxin

• Uncharacterized protein HI1420 (4-96)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12604.47 Da
• Isoelectric Point: 8.9594

>T22760 WP_006479524.1 NC_010515:654839-655177 [Burkholderia cenocepacia MC0-3]
MVDILQSVPYSAPTFSIRTTDVFEAWFAGLPDRVARRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLCGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T22760 NC_010515:654839-655177 [Burkholderia cenocepacia MC0-3]
ATGGTTGACATCCTGCAATCCGTGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGAAGCGTGGTT
CGCCGGCCTGCCGGATCGCGTCGCGCGGCGGCGCATCCAGGCGCGCATCGACCGTCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCCGGCTCCCCCGTCGTCGAAATGCGTATCGACCACGGCCCCGGCTATCGCGTCTATTACGTGAGGCGCGGA
ACGATATGGGTCATCCTGCTCTGCGGCGGCGATAAGTCGACGCAACAGGCCGACATCCGCGCCGCGCACGCAATGCTCGC
GCACCTCGACATGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10608.04 Da
• Isoelectric Point: 6.4771

>AT22760 WP_006490972.1 NC_010515:655181-655480 [Burkholderia cenocepacia MC0-3]
MDKIKTRPWDSAEHLKTEDDMADYFDACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAAA

Nucleotide

• Length: 300 bp

>AT22760 NC_010515:655181-655480 [Burkholderia cenocepacia MC0-3]
ATGGACAAGATCAAGACGCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGAAGACGACATGGCCGACTACTTCGACGC
CTGCCTGCAGGAGGCCGGCGACGATCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGTGCCCGCGGCATGTCGC
AGGTCGCCCGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAATTGCACGGCGCCAAGGCTGCCGCGTGA

Structures

Toxin

• AlphaFold DB: B1K4X0