Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Acetyltransf_4(toxin)
Location 5460328..5461836 Replicon chromosome
Accession NZ_CP089065
Organism Pseudomonas aeruginosa strain UNC_PaerCF34

Toxin (Protein)


Gene name - Uniprot ID Q9HYX1
Locus tag LSP20_RS25400 Protein ID WP_003119682.1
Coordinates 5461249..5461836 (-) Length 196 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A1C7C4U7
Locus tag LSP20_RS25395 Protein ID WP_003091607.1
Coordinates 5460328..5461182 (-) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSP20_RS25380 (LSP20_25385) 5455747..5455956 + 210 WP_003115568.1 cold-shock protein -
LSP20_RS25385 (LSP20_25390) 5456017..5457891 - 1875 WP_003117607.1 MFS transporter -
LSP20_RS25390 (LSP20_25395) 5458066..5460231 - 2166 WP_003091606.1 TonB-dependent receptor family protein -
LSP20_RS25395 (LSP20_25400) 5460328..5461182 - 855 WP_003091607.1 AraC family transcriptional regulator Antitoxin
LSP20_RS25400 (LSP20_25405) 5461249..5461836 - 588 WP_003119682.1 GNAT family protein Toxin
LSP20_RS25405 (LSP20_25410) 5462102..5465581 + 3480 WP_003117605.1 PAS domain-containing hybrid sensor histidine kinase/response regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(20-152)

Antitoxin

AraC-type arabinose-binding/dimerisation domain

(19-151)


Sequences

Toxin        


Download         Length: 196 a.a.        Molecular weight: 21901.23 Da        Isoelectric Point: 9.8676

>T227599 WP_003119682.1 NZ_CP089065:c5461836-5461249 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Download         Length: 588 bp

>T227599 NZ_CP123001:307256-307753 [Neorhizobium petrolearium]
GTGACCCTCAGCGCACCGGCACCCCTGGCCGAACATCACGAGCTGGCTGAATTCAATTCCGGCGTCGCCGAACTGGACGA
CTGGCTGCGACGCCGCGCTCGTTCTAATCAAGCAAGCGGGGCATCGCGGACCTTCGTCGTGTGCGACGAGAGCCGCGTCA
TCGCCTATTACGCGCTCGCCTCCGGCGCCGTGAAACAGCCGGAAGCGCCTGGCCGTTTTCGGCGCAATATGCCTGATCCA
ATTCCGGTCGCTGTGCTTGGTCGCCTCGCTATTGATCAATCCTACCAGGGGCGAGGTATCGGCAGGGCATTGGTGCGCGA
CGCGGGCCTACGCCTGCTCAATGCCGCGGAAGTACTTGGCATTCGAGGTATGCTCGTTCATGCGATTTCAGATGACGCAC
GAGCCTTCTACGAGGCCGTCGGCTTCCTGTCTTCTCCTTCCGATCCAATGATGCTGATGGTCGGATTGCATGACCTCAAT
AACGCGCTGAACCCTTAG

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31895.20 Da        Isoelectric Point: 7.5295

>AT227599 WP_003091607.1 NZ_CP089065:c5461182-5460328 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETMLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Download         Length: 855 bp

>AT227599 NZ_CP123001:306963-307259 [Neorhizobium petrolearium]
ATGCCAACCTCACACACGAGCAAAAGCTCGGACGCAAGCGTGCCGCTGAATATGCGCATCAAGCCCGCGACCCGCAACTT
GATTGATCGAGCCGCTGAGTTGCTTGGTAAGACGCGCACGGATTTCATGTTGGAGGCTTCCGAGCGCCGAGCCGAGGAAG
TACTCCTCGACCGCACTATCTTTACGGTAGGCCCGGAAATCTATGCTGAATACCTTGCCCGGCTCGATGCACCTACGCAG
CCCAATGAGCGTTTGACGCGCACGATGTCGACCAAAGCGCCGTGGGATGAGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1YRE
PDB 8GXJ

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7C4U7

References