Detailed information of TA system TA227594

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 570773..571278
• Replicon: chromosome
• Accession: NZ_CP089065
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF34

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LSP20_RS02730
• Protein ID: WP_023518248.1
• Coordinates: 570997..571278 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: LSP20_RS02725
• Protein ID: WP_003083775.1
• Coordinates: 570773..571000 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP20_RS02700 (LSP20_02700)	565791..567284	+	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-
LSP20_RS02705 (LSP20_02705)	567453..568880	+	1428	WP_003083784.1	GABA permease	-
LSP20_RS02710 (LSP20_02710)	568962..569303	-	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
LSP20_RS02715 (LSP20_02715)	569376..569876	-	501	WP_003101228.1	LEA type 2 family protein	-
LSP20_RS02720 (LSP20_02720)	569977..570597	+	621	WP_003101226.1	hypothetical protein	-
LSP20_RS02725 (LSP20_02725)	570773..571000	+	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
LSP20_RS02730 (LSP20_02730)	570997..571278	+	282	WP_023518248.1	type II toxin-antitoxin system toxin ParE	Toxin
LSP20_RS02735 (LSP20_02735)	571578..572486	+	909	WP_231822276.1	LysR family transcriptional regulator	-
LSP20_RS02740 (LSP20_02740)	572518..572928	-	411	WP_003101225.1	aegerolysin family protein	-
LSP20_RS02745 (LSP20_02745)	573108..573842	-	735	WP_003117208.1	GntR family transcriptional regulator	-
LSP20_RS02750 (LSP20_02750)	573943..574629	-	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
LSP20_RS02755 (LSP20_02755)	574678..576027	-	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10472.23 Da
• Isoelectric Point: 10.0435

>T227594 WP_023518248.1 NZ_CP089065:570997-571278 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLPLERWPFSAPFRVKGKE
IQILRIDRIEITP

Nucleotide

• Length: 282 bp

>T227594 NZ_CP123000:c2760504-2760064 [Neorhizobium petrolearium]
TTGTATCTTCTGGACACCAATATTGTGTCGGCCGATGCGCCGACCAAGCGCCATGTCGGTGTCGAGGCGTTCGCGGCGTG
GGTGCGTGCGAATAGTGACAGGCTGTATCTTTCGGCCATCACCATCGCCGAGATCGAGGCGGGCATTGCCCGCGCCGTCA
GGATCGAGGCGACGACGAAGGCGACAAACCTGCGCCGCTGGCTTGCCGCCGTCGAACATTTCTATGCCGGTCGTATTCTA
CCGTTCGGCGTCGAGGAGGCCAGACATGCGGGCCTGATGCTCGATCGGGCGCGAGCTCATGATCCCGGTTTCGAGGACAT
CGCCATTGCCGCAACCGCCGCCGCGCATGGTCTCACGGTTCTGACGGCTAATGAGCGTCATTTCGAGCCACTGGGCGTGC
CACTCGCCAATCCCCTGAAGCAATTGCCGACCATTCAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT227594 WP_003083775.1 NZ_CP089065:570773-571000 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT227594 NZ_CP123000:c2760758-2760507 [Neorhizobium petrolearium]
ATGAAGGAAATCCAGCTCAAGGACGCGAAGGCTACGCTTTCCGCCGTGGTCGATCGAGCCGTGGCCGGCGAGCCGACCGT
TATCACCCGTCATGGTCGCAAGGAAGCCGTGCTGGTTTCCTTCGAGGAGTGGGAACGAGTTTCCAAGGTGCCGGACTTTG
CCGATCTGCTTTTAGCCTTTCCCGGTGAGCCGGAGGATATTCCCGGACGATCGGGGAAGCCCGCCCGCGCATTGCGCGAA
AACGGTCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1C7BDS9