Detailed information of TA system TA227592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 2963117..2963622
• Replicon: chromosome
• Accession: NZ_CP089064
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF35

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: LSP22_RS13735
• Protein ID: WP_003083773.1
• Coordinates: 2963117..2963398 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: LSP22_RS13740
• Protein ID: WP_003083775.1
• Coordinates: 2963395..2963622 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP22_RS13710 (LSP22_13715)	2958368..2959717	+	1350	WP_231822170.1	C4-dicarboxylate transporter DctA	-
LSP22_RS13715 (LSP22_13720)	2959766..2960452	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
LSP22_RS13720 (LSP22_13725)	2960553..2961287	+	735	WP_003117208.1	GntR family transcriptional regulator	-
LSP22_RS13725 (LSP22_13730)	2961467..2961877	+	411	WP_003101225.1	aegerolysin family protein	-
LSP22_RS13730 (LSP22_13735)	2961909..2962817	-	909	WP_003083769.1	LysR family transcriptional regulator	-
LSP22_RS13735 (LSP22_13740)	2963117..2963398	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
LSP22_RS13740 (LSP22_13745)	2963395..2963622	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
LSP22_RS13745 (LSP22_13750)	2963798..2964418	-	621	WP_003101226.1	hypothetical protein	-
LSP22_RS13750 (LSP22_13755)	2964519..2965019	+	501	WP_003112629.1	LEA type 2 family protein	-
LSP22_RS13755 (LSP22_13760)	2965092..2965433	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
LSP22_RS13760 (LSP22_13765)	2965515..2966942	-	1428	WP_003083784.1	GABA permease	-
LSP22_RS13765 (LSP22_13770)	2967111..2968604	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T227592 WP_003083773.1 NZ_CP089064:c2963398-2963117 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T227592 NZ_CP123000:c2541389-2541108 [Neorhizobium petrolearium]
GTGATTCGATCGTTCAGGGACAAGCTCACTCAATCGATCGATGACGGCACGGTCAGGAAAGGTTTCCCTTCCGATCTGGT
TCGTCGCGCCCAACAATTGCTGACTGTCTTAAATGCGGCAACATCTTTGGAAGACCTTCGCTCTCCACCGGGTAACCGGT
TGGAAAAGCTCTCCGGAGATCGAGAAGGCCAGCATTCAATCCGCATCAACAAGCAGTGGCGGATTTGCTTCGTCTGGACT
GAAGCCGGGCCGGGAGAGGTGGAAATCACTGATTACCATTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT227592 WP_003083775.1 NZ_CP089064:c2963622-2963395 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT227592 NZ_CP123000:c2541080-2540778 [Neorhizobium petrolearium]
ATGAGTGTCAGCAAACTGCCGCCTATTCATCCCGGTGAGATTCTATGCGACCTCTATCTCAGGCCTTTGGAGATGACCCC
ATATGCGTTGGCCAAGAAGCTGAACGTACCGCGCACGCGTATTGAACGCATTGTTGCCCAGAAGACCGGCATCACGTCGG
ATACCGCTCTGCGCCTGGCCAGATTCTTCAGGACTTCGCCGGAATTCTGGATGAATCTTCAAAACAGCTTCGATCTGAAG
ATCGAGGCGCAGGCTCTGAAAGATGAACTGAAGCGCATTCCGGAATACGAGGCTGCTGCTTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9