Detailed information of TA system TA022759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 295286..295863
• Replicon: chromosome
• Accession: NC_010515
• Organism: Burkholderia cenocepacia MC0-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B1K205
• Locus tag: BCENMC03_RS17365
• Protein ID: WP_012338377.1
• Coordinates: 295498..295863 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1K204
• Locus tag: BCENMC03_RS17360
• Protein ID: WP_011547971.1
• Coordinates: 295286..295504 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCENMC03_RS17340	291148..292404	-	1257	WP_012338373.1	Zn-dependent hydrolase	-
BCENMC03_RS17345	292397..292921	-	525	WP_012338374.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
BCENMC03_RS17350	292953..294119	-	1167	WP_012338375.1	branched-chain amino acid ABC transporter substrate-binding protein	-
BCENMC03_RS17355	294298..295194	+	897	WP_012338376.1	LysR family transcriptional regulator	-
BCENMC03_RS17360	295286..295504	+	219	WP_011547971.1	hypothetical protein	Antitoxin
BCENMC03_RS17365	295498..295863	+	366	WP_012338377.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCENMC03_RS17370	296159..296722	+	564	WP_011547973.1	peroxiredoxin	-
BCENMC03_RS17375	296841..298442	+	1602	WP_012338378.1	alkyl hydroperoxide reductase subunit F	-
BCENMC03_RS17380	298537..299349	-	813	WP_012338379.1	hypothetical protein	-
BCENMC03_RS17385	299505..300404	-	900	WP_012338380.1	lipid A hydroxylase LpxO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13424.49 Da
• Isoelectric Point: 8.4487

>T22759 WP_012338377.1 NC_010515:295498-295863 [Burkholderia cenocepacia MC0-3]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISMISWMEVLVGASVSDEAPIRAWLSSFNVIALDSTVANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPASEPGVRVPYQI

Nucleotide

• Length: 366 bp

>T22759 NC_010515:295498-295863 [Burkholderia cenocepacia MC0-3]
ATGGTAAAAGCCCTTTTCGACACCAACATCCTGATCGATTATCTGGGCGGCGTGGAATCCGCGCGTGCGGAGCTAGGCCG
CTACGACTATCGCGCAATCAGCATGATCTCGTGGATGGAAGTCCTCGTCGGGGCGTCCGTAAGTGACGAGGCCCCCATCC
GTGCATGGCTCTCGTCGTTCAATGTCATCGCGCTCGACAGCACGGTGGCGAATCGCGCCGTGACGATTCGCAAGGAACGC
CGCATACGCCTGCCCGACGCAATCGTGTGGGCGTCGGCACAAGTGAACGGGCTGTTGCTCGTATCGCGCAACACGAAGGA
CTTTCCCGCCTCCGAGCCCGGCGTTCGCGTGCCATATCAGATCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8292.38 Da
• Isoelectric Point: 4.5560

>AT22759 WP_011547971.1 NC_010515:295286-295504 [Burkholderia cenocepacia MC0-3]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALNKHRLADDVFGLWKDRTVDGLAYQEELRSEW

Nucleotide

• Length: 219 bp

>AT22759 NC_010515:295286-295504 [Burkholderia cenocepacia MC0-3]
ATGAGCCGGATTCTGGTCGATCTGTCGGATGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACACCGTCCCCG
CGCCGCCATCATTCGTGACGCGATCGACGCCTACATTGCCCTGAACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTAT
GGAAAGATCGCACGGTCGACGGGCTCGCCTATCAAGAGGAATTGCGCTCGGAATGGTAA

Structures

Toxin

• AlphaFold DB: B1K205

Antitoxin

• AlphaFold DB: B1K204