Detailed information of TA system TA227589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 933094..934351
• Replicon: chromosome
• Accession: NZ_CP089064
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF35

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LSP22_RS04385
• Protein ID: WP_003449003.1
• Coordinates: 933094..933849 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: LSP22_RS04390
• Protein ID: WP_003086329.1
• Coordinates: 933983..934351 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP22_RS04375 (LSP22_04375)	928267..930810	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
LSP22_RS04380 (LSP22_04380)	930965..932326	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
LSP22_RS04385 (LSP22_04385)	933094..933849	-	756	WP_003449003.1	RES family NAD+ phosphorylase	Toxin
LSP22_RS04390 (LSP22_04390)	933983..934351	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
LSP22_RS04395 (LSP22_04395)	934424..935710	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
LSP22_RS04400 (LSP22_04400)	935766..937259	-	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
LSP22_RS04405 (LSP22_04405)	937434..937910	+	477	WP_003112531.1	hypothetical protein	-
LSP22_RS04410 (LSP22_04410)	937913..938188	-	276	WP_010792073.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28292.45 Da
• Isoelectric Point: 7.7294

>T227589 WP_003449003.1 NZ_CP089064:c933849-933094 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T227589 NZ_CP123000:505067-505390 [Neorhizobium petrolearium]
ATGCAAAGAGGGGATCTCGTCACGGTCGCCTTAACCGGCGATTTCGGCAAGCCGCGCCCCGCGCTCATAGTCCAATCTGA
TCTGTTCGACGAGACGGACACCCTTACAGTTTTGCTGCTTTCCAGCACGCTGGTGAATGCGCCCCTTGTTCGCCTTACAG
TGCAGCCAGATGCCACGAATGCGCTTAACAAGCCTTCGCAGATCATGGTCGACAAGACGATGACTGTAAGGCGCGATAAA
CTGGGAAAAGTCTTCGGCCGTATTGATGATGAGACGATGATTTCCGTGACCCGGTCACTTGCTGTGTTTTTCGGATTCGC
GTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT227589 WP_003086329.1 NZ_CP089064:c934351-933983 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT227589 NZ_CP123000:504852-505067 [Neorhizobium petrolearium]
ATGCCTTCTTCGGTAAGTGAGCGCGTCAGGAAGCGTCGCGAAAGTCTTCGGGCGGCCGGTTTGCGGCCACTGCAAATTTG
GGTGCCTGATACGCGACGTCCAGGTTTTGCAGAAGAGTGCCGCCGCCAATCGAGAGTGGTCGCAGCAGCGGACGCTGCAG
ATCCCGATCTGCTGAATTTTCTGGATGAGGCTCTGGCAGATCTCGGTGAACCGTGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7