Detailed information of TA system TA227588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 25998..27219
• Replicon: chromosome
• Accession: NZ_CP089064
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF35

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: LSP22_RS00135
• Protein ID: WP_003102740.1
• Coordinates: 26641..27219 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LSP22_RS00130
• Protein ID: WP_003088112.1
• Coordinates: 25998..26552 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP22_RS00105 (LSP22_00105)	21566..22117	+	552	WP_034074903.1	XRE family transcriptional regulator	-
LSP22_RS00110 (LSP22_00110)	22114..22512	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
LSP22_RS00115 (LSP22_00115)	22525..22848	-	324	WP_003162861.1	multidrug efflux SMR transporter	-
LSP22_RS00120 (LSP22_00120)	23179..23640	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
LSP22_RS00125 (LSP22_00125)	23645..25840	+	2196	WP_004343108.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
LSP22_RS00130 (LSP22_00130)	25998..26552	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
LSP22_RS00135 (LSP22_00135)	26641..27219	+	579	WP_003102740.1	HD domain-containing protein	Toxin
LSP22_RS00140 (LSP22_00140)	27329..28516	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
LSP22_RS00145 (LSP22_00145)	28506..30677	-	2172	WP_003088105.1	type I secretion system permease/ATPase	-
LSP22_RS00150 (LSP22_00150)	30667..31944	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T227588 WP_003102740.1 NZ_CP089064:26641-27219 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T227588 NZ_CP123000:c412468-412199 [Neorhizobium petrolearium]
TTGGCCTGGACGATTGAGTTACGGGATAGCGCGCGGAAGCAACTAAAGCATCTCGCCCACCACGATGCAGCGCGCATTAT
CTCATTTCTTGAAGAGCGTGTCGCGACACACGAAAATCCCAGAGAACTTGGCGCGGCGCTGAAAGGGCACGTCCTTGGCG
GGTACTGGCGCTACCGCGTCGGCGACTATCGCATCATCTGCGAAATCCAGGATCAGCGGCTCGTCGTGCTGGTGATCGAG
ATCGGTCATCGGCGCGAAGTCTATCGCTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT227588 WP_003088112.1 NZ_CP089064:25998-26552 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT227588 NZ_CP123000:c412682-412452 [Neorhizobium petrolearium]
ATGAACAAACGCGTCACGATCGAAATGCCGGACGAAATGCACAAGCTGATCGCCGAATACGCCGCGGAAGCCGGTGCCGA
GCCGGATACCTATATGCGCGACGTGCTGGAACAGCATCTCGAAGATCTGCATGACATTGCGCTTGCGGAAGCCGCAATGG
AGCGGATTCGCAACGGCGAGCGGACCTATACACTGGAAGAAGTGAAGCGCGAACTTGGCCTGGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07