Detailed information of TA system TA227587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 6574690..6575195
• Replicon: chromosome
• Accession: NZ_CP089063
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF37

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: LSP18_RS30715
• Protein ID: WP_003083773.1
• Coordinates: 6574914..6575195 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: LSP18_RS30710
• Protein ID: WP_003083775.1
• Coordinates: 6574690..6574917 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP18_RS30685 (LSP18_30945)	6569708..6571201	+	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-
LSP18_RS30690 (LSP18_30950)	6571370..6572797	+	1428	WP_003083784.1	GABA permease	-
LSP18_RS30695 (LSP18_30955)	6572879..6573220	-	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
LSP18_RS30700 (LSP18_30960)	6573293..6573793	-	501	WP_003112629.1	LEA type 2 family protein	-
LSP18_RS30705 (LSP18_31845)	6573894..6574514	+	621	WP_003101226.1	hypothetical protein	-
LSP18_RS30710 (LSP18_30970)	6574690..6574917	+	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
LSP18_RS30715 (LSP18_30975)	6574914..6575195	+	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
LSP18_RS30720 (LSP18_30980)	6575495..6576403	+	909	WP_057383068.1	LysR family transcriptional regulator	-
LSP18_RS30725 (LSP18_30985)	6576435..6576845	-	411	WP_003101225.1	aegerolysin family protein	-
LSP18_RS30730 (LSP18_30990)	6577025..6577759	-	735	WP_003117208.1	GntR family transcriptional regulator	-
LSP18_RS30735 (LSP18_30995)	6577860..6578546	-	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
LSP18_RS30740 (LSP18_31000)	6578595..6579944	-	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T227587 WP_003083773.1 NZ_CP089063:6574914-6575195 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T227587 NZ_CP122997:84413-84715 [Mycolicibacterium aubagnense]
GTGATCGCTCCCGGCGATATTTGCCCACGTCGCGACACCAGCCATGAGTTTTACGTTGTGGTGCTGTCCAACTCGATTCA
CTTGGCCGCCGATACCGGGCGGGTCATCACCTGCCCCTATATTCCGGGCCAAATCCCTGACGCTGCTATGGCGCTCGTCG
TTCGAGTAGAGCAGCCCGAAGGTGTGGCTCTCCCGGAGCTCGTTCAGTGGCTTCCCACTGCCGCACTCGATGAGCCGATC
GGCAACATCGACATCACCGCGCTGCGTGCAACAACGGGCCTCGTTACCGCTCTGGTCACCTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT227587 WP_003083775.1 NZ_CP089063:6574690-6574917 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT227587 NZ_CP122997:84189-84416 [Mycolicibacterium aubagnense]
ATGGCGAAAGATAAGGTGACCGTGACTGTGGATCAAGAGGTGCTGGCCGCGGCAGACGCGGACGCTAAGGCGGCCGGGCT
CAACCGATCCGAGCTGGTGGAGCGGGCCTTACGTCACGAACATCTGCGGATCGCGCTCAAGAGCTACACGGCCGAGACTG
TTCCGGCGCTGAATATCGACGACTACGCGCAGAACATCTACGAGGCGAACCGGGCCGCAGGTCTGTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9