Detailed information of TA system TA227578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 4763455..4764676
• Replicon: chromosome
• Accession: NZ_CP089062
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF38

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: LSP17_RS21825
• Protein ID: WP_003102740.1
• Coordinates: 4764098..4764676 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LSP17_RS21820
• Protein ID: WP_003088112.1
• Coordinates: 4763455..4764009 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP17_RS21795 (LSP17_21795)	4759023..4759574	+	552	WP_003450159.1	XRE family transcriptional regulator	-
LSP17_RS21800 (LSP17_21800)	4759571..4759969	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
LSP17_RS21805 (LSP17_21805)	4759982..4760305	-	324	WP_023119961.1	multidrug efflux SMR transporter	-
LSP17_RS21810 (LSP17_21810)	4760636..4761097	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
LSP17_RS21815 (LSP17_21815)	4761102..4763297	+	2196	WP_004349295.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
LSP17_RS21820 (LSP17_21820)	4763455..4764009	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
LSP17_RS21825 (LSP17_21825)	4764098..4764676	+	579	WP_003102740.1	HD domain-containing protein	Toxin
LSP17_RS21830 (LSP17_21830)	4764785..4765972	-	1188	WP_061189744.1	HlyD family efflux transporter periplasmic adaptor subunit	-
LSP17_RS21835 (LSP17_21835)	4765962..4768133	-	2172	WP_070654195.1	type I secretion system permease/ATPase	-
LSP17_RS21840 (LSP17_21840)	4768123..4769400	-	1278	WP_073669307.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T227578 WP_003102740.1 NZ_CP089062:4764098-4764676 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T227578 NZ_CP122987:3363507-3363803 [Aeromonas salmonicida]
ATGAGAGAGATAATCAAAACAAACGTGTTCGACCGCTGGTTTGACTCCCTGCGAGATGCTCGCGCACAGGCAAAGGTGAC
AGCCAGGATCCGGCGCTTGGGTCTTGGCAATCCTGGGGACGTTAAACCTATCGGGGAGGGTCTATCGGAAATGCGGATAG
ACTACGGCCCCGGGTACAGAGTGTATTTTATGACAAAAGGCCCGATCATCATCGTGCTGCTGTGCGGTGGGGATAAGGGC
ACCCAAGCCCGTGATATCGAACAGGCAAAAACCATTGCCGCGCAATGGAAGGATTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT227578 WP_003088112.1 NZ_CP089062:4763455-4764009 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT227578 NZ_CP122987:3363806-3364126 [Aeromonas salmonicida]
ATGAAAGACCATGAGCAGACCGTCACATTCAGTCGTTATGATGCGGCTGACTACCTCAAGACCGATGAAGACATGCTCCA
CTATTTGGAGGCCGTCATGGAAGAGGGCGATCCCGCCTTGATTGCAGCCGCCCTCGGTGACATCGCCCGCGCTCGCAACC
TGAGCCAGCTGGCCCGTGATGTTGGGATGAGCAGAGAGGGGCTATACAAAGCCTTATCCGGAGAAGGCAACCCGACCTTT
GCCACCATAACCAAAGTGGCGGGCGCACTGGGGCTGCGCTTTGGTGTCCACCTTGCCAATCCGCCAGCAACCCACGCGTG
A

Structures

Toxin

• AlphaFold DB: A0A0C7CS07