Detailed information of TA system TA227571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 3048708..3049213
• Replicon: chromosome
• Accession: NZ_CP089061
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF41

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: LSP19_RS14020
• Protein ID: WP_003083773.1
• Coordinates: 3048708..3048989 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: LSP19_RS14025
• Protein ID: WP_003083775.1
• Coordinates: 3048986..3049213 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP19_RS13995 (LSP19_14000)	3043959..3045308	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
LSP19_RS14000 (LSP19_14005)	3045357..3046043	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
LSP19_RS14005 (LSP19_14010)	3046144..3046878	+	735	WP_003117208.1	GntR family transcriptional regulator	-
LSP19_RS14010 (LSP19_14015)	3047058..3047468	+	411	WP_003110659.1	aegerolysin family protein	-
LSP19_RS14015 (LSP19_14020)	3047500..3048408	-	909	WP_003083769.1	LysR family transcriptional regulator	-
LSP19_RS14020 (LSP19_14025)	3048708..3048989	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
LSP19_RS14025 (LSP19_14030)	3048986..3049213	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
LSP19_RS14030 (LSP19_14035)	3049389..3050009	-	621	WP_034004118.1	hypothetical protein	-
LSP19_RS14035 (LSP19_14040)	3050110..3050610	+	501	WP_034004120.1	LEA type 2 family protein	-
LSP19_RS14040 (LSP19_14045)	3050683..3051024	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
LSP19_RS14045 (LSP19_14050)	3051106..3052533	-	1428	WP_003083784.1	GABA permease	-
LSP19_RS14050 (LSP19_14055)	3052702..3054195	-	1494	WP_016263977.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T227571 WP_003083773.1 NZ_CP089061:c3048989-3048708 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T227571 NZ_CP122961:702467-702745 [Halomonas alkaliantarctica]
ATGATCCGAAGCTTCAAACACAAAGGTTTGGCAAAGTTCTTCAAATCCGGCAGCACTGCAGGGATTCAGGCCGCTCATGC
AAAGAGGCTTCGGCTTATTCTTGGTCGGCTCAATGCTGCGTCAGATACCAAAGATATGGACTTACCAGGCCTTCGGTTGC
ACGAACTCTCAGGAAATCGCTCTGGTATCTGGTCAGTCACGGTTAGTGGTAACTGGCGAGTGACGTTTCGGTTTGAGGAC
GGAGATGCCGAGATAGTGAATTATGAAGATTACCACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT227571 WP_003083775.1 NZ_CP089061:c3049213-3048986 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT227571 NZ_CP122961:702756-703043 [Halomonas alkaliantarctica]
ATGTTGATGCACAATCCTCCCCACCCTGGTGAAGTATTACGCGAGCTTTGCTTGGAGCCCTTAGGTCTTACTGTGACAGC
CGCAGCGGAAGCGCTGGGAGTAAGCCGCAAAACGCTGAGTGCTGTCCTGAACGGCAAGGCCGGTATTAGCCCTGAAATGG
CGGTGCGGCTTTCTATCGCGTTTGATACCTCTGCGGAAAGCTGGCTGAACCAGCAGTCCCAATACGAACTCTGGCACGCC
GAACAGCATCGTAAAGAGCTTAAAGTAAAGCGGCTGGTTGCCGCCTAA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9