Detailed information of TA system TA227569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 968666..969924
• Replicon: chromosome
• Accession: NZ_CP089061
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF41

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LSP19_RS04540
• Protein ID: WP_003163133.1
• Coordinates: 968666..969421 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: LSP19_RS04545
• Protein ID: WP_003086329.1
• Coordinates: 969556..969924 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSP19_RS04530 (LSP19_04530)	963838..966381	+	2544	WP_231786403.1	acyl-homoserine-lactone acylase QuiP	-
LSP19_RS04535 (LSP19_04535)	966536..967897	-	1362	WP_073657578.1	DNA recombination protein RmuC	-
LSP19_RS04540 (LSP19_04540)	968666..969421	-	756	WP_003163133.1	RES family NAD+ phosphorylase	Toxin
LSP19_RS04545 (LSP19_04545)	969556..969924	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
LSP19_RS04550 (LSP19_04550)	969997..971283	-	1287	WP_029611025.1	FAD-binding oxidoreductase	-
LSP19_RS04555 (LSP19_04555)	971339..972832	-	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
LSP19_RS04560 (LSP19_04560)	973007..973483	+	477	WP_029611026.1	hypothetical protein	-
LSP19_RS04565 (LSP19_04565)	973486..973761	-	276	WP_172655204.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T227569 WP_003163133.1 NZ_CP089061:c969421-968666 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T227569 NZ_CP122956:587752-588018 [Helicobacter pylori]
GTGTTGAAACTCAACCTTAAAAAATCTTTCCAAAAAGATTTTGATAAATTACTTTTGAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAACCTTAAGAAAAAAAGAACCATTAGACCCACAATTTCAAGATCATGCTTTAAAGGGAAAGTGGA
AACCTTTTAGGGAATGCCACATCAAAGCTGATATTTTGCTTGTGTATCTAGTGAAAGATGATGAGCTTGTTTTGTTAAGA
CTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT227569 WP_003086329.1 NZ_CP089061:c969924-969556 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT227569 NZ_CP122956:588097-588408 [Helicobacter pylori]
ATGCCCAACACCACCGCAGAAAAAGACTACACCCAATACAGCGAAAAACAGCTTTTTAACTTTTTAAATTCTATCAAGAC
CAAACAAAAAAGAGCGTTAGAAAAACTGAAAGAAATCCAAGCTCAAAAACAAAGAATCAAAAAAGCGCTCCAATTTAAAG
CGCTCCATTTAACCGAAAACGGATACACCATAGAAGAAGAGCGAGAAATCTTAGCAAGGGCTAAAGACACCAAGAACCAC
CTTTGTTTTAAAAGCGTAGAGGATTTTAAAAAGCATTGTGAAAACTTAAGATTTAAAAATGCTGATGATTGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7