Detailed information of TA system TA227566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): AbkB-brnA/BrnT-BrnA
• Location: 296603..297191
• Replicon: plasmid pRIVM_C010559_1
• Accession: NZ_CP089052
• Organism: Acinetobacter ursingii strain RIVM_C010559

Toxin (Protein)

• Gene name: AbkB
• Uniprot ID: -
• Locus tag: LSO60_RS17665
• Protein ID: WP_130114512.1
• Coordinates: 296904..297191 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: LSO60_RS17660
• Protein ID: WP_130114513.1
• Coordinates: 296603..296917 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSO60_RS17640 (LSO60_17600)	294219..294583	+	365	Protein_285	copper-binding protein	-
LSO60_RS17645 (LSO60_17605)	294775..295478	-	704	Protein_286	IS3 family transposase	-
LSO60_RS17650 (LSO60_17610)	295542..296247	+	706	Protein_287	IS6-like element IS1006 family transposase	-
LSO60_RS17655 (LSO60_17615)	296299..296526	+	228	Protein_288	ISNCY family transposase	-
LSO60_RS17660 (LSO60_17620)	296603..296917	-	315	WP_130114513.1	BrnA antitoxin family protein	Antitoxin
LSO60_RS17665 (LSO60_17625)	296904..297191	-	288	WP_130114512.1	BrnT family toxin	Toxin
LSO60_RS17670 (LSO60_17630)	297421..298026	-	606	WP_000447788.1	LysE family translocator	-
LSO60_RS17675 (LSO60_17635)	298076..298495	-	420	WP_263503917.1	AraC family transcriptional regulator	-
LSO60_RS17685 (LSO60_17645)	299838..300188	-	351	Protein_294	AraC family ligand binding domain-containing protein	-
LSO60_RS17690 (LSO60_17650)	300362..301068	-	707	Protein_295	IS1 family transposase	-
LSO60_RS17695 (LSO60_17655)	301159..302001	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / blaCARB-2 / sul1 / dfrA19 / blaIMP-4 / catB3 / ARR-3 / qacE / aph(3')-VIa / msr(E) / mph(E) / blaOXA-58 / aac(3)-IId	-	1..313387	313387
	-	inside	IScluster/Tn	blaOXA-58 / aac(3)-IId	-	294994..313299	18305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11262.71 Da
• Isoelectric Point: 6.0900

>T227566 WP_130114512.1 NZ_CP089052:c297191-296904 [Acinetobacter ursingii]
MKQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T227566 NZ_CP122955:652380-652661 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATTTTATCTGGGCGTATTACAGAAAACGA
TGTTACAAGTATTGTTGATTGCCTAAAAAAACAAAAACCACTCCAACAAAAATATTGCGACCATGCTTTGAGTGGCAATC
TTAAAGGGTTGAGAGAATGCCATGTCAAACCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12254.89 Da
• Isoelectric Point: 7.4024

>AT227566 WP_130114513.1 NZ_CP089052:c296917-296603 [Acinetobacter ursingii]
MSMVRHSRKELNEKFSDKQDAEIERLLAKGTVPDDHLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT227566 NZ_CP122955:652073-652387 [Helicobacter pylori]
ATGTCAAATGTTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAAGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAGG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACCAAGTTTGTTAAATACGATCGCTTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTGCAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA