Detailed information of TA system TA227564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 254749..255333
• Replicon: plasmid pRIVM_C010559_1
• Accession: NZ_CP089052
• Organism: Acinetobacter ursingii strain RIVM_C010559

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: LSO60_RS17445
• Protein ID: WP_000286964.1
• Coordinates: 254749..255051 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LSO60_RS17450
• Protein ID: WP_000985609.1
• Coordinates: 255052..255333 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSO60_RS17425 (LSO60_17385)	250099..251424	-	1326	WP_081313053.1	ISNCY family transposase	-
LSO60_RS17430 (LSO60_17390)	251576..252454	-	879	WP_004973252.1	IS982-like element ISAba4 family transposase	-
LSO60_RS17435 (LSO60_17395)	252584..254038	-	1455	WP_026438532.1	SulP family inorganic anion transporter	-
LSO60_RS17440 (LSO60_17400)	254288..254497	-	210	WP_004976362.1	hypothetical protein	-
LSO60_RS17445 (LSO60_17405)	254749..255051	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSO60_RS17450 (LSO60_17410)	255052..255333	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
LSO60_RS17455 (LSO60_17415)	255413..255862	-	450	WP_228719708.1	SMI1/KNR4 family protein	-
LSO60_RS17460 (LSO60_17420)	255897..257028	+	1132	Protein_249	IS3-like element ISAba14 family transposase	-
LSO60_RS17465 (LSO60_17425)	257295..258619	+	1325	Protein_250	ISNCY family transposase	-
LSO60_RS17470 (LSO60_17430)	258744..259064	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LSO60_RS17475 (LSO60_17435)	259057..259329	+	273	WP_000369781.1	NadS family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / blaCARB-2 / sul1 / dfrA19 / blaIMP-4 / catB3 / ARR-3 / qacE / aph(3')-VIa / msr(E) / mph(E) / blaOXA-58 / aac(3)-IId	-	1..313387	313387
	-	inside	IScluster/Tn	msr(E) / mph(E)	-	250099..264257	14158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T227564 WP_000286964.1 NZ_CP089052:254749-255051 [Acinetobacter ursingii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T227564 NZ_CP122952:557475-557735 [Helicobacter pylori]
ATGCTGAAAATTAGGCATCATATCTTTTTCAAAAAAGACTTTAAAAAGCTTGTTAAAAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAAGAAAAAAAGAACCGCTAGATCCACAATTTCAAGATCATGCCCTAAAAGGCAAGTGGAAACCTT
TTAGGGAATGCCACATCAAAGCTGATGTTTTGCTTGTGTATTTAGTGCGAGATGATGAGCTTGTTTTGTTAAGGTTAGGC
AGTCATAGCGAGCTGTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT227564 WP_000985609.1 NZ_CP089052:255052-255333 [Acinetobacter ursingii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT227564 NZ_CP122952:557117-557488 [Helicobacter pylori]
ATGCCTAATTCTCCCACCAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAATTAGAGCA
AAAAATCAAAAAGATGCAAAATGATAGAGCTTCTTTCAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCATTAAATGGACTCTTGCAAAAAATCAGCCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGTTAAATGGCGAAAACTTAGAAACTATTGAAGA
CTTTTCTGCATGGGCAAATGAAGTGAAAAAGGAAATGAATGCTGAAAATTAG

Structures

Toxin

• AlphaFold DB: A0A0A8TV94