Detailed information of TA system TA227563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 249414..250002
• Replicon: plasmid pRIVM_C010559_1
• Accession: NZ_CP089052
• Organism: Acinetobacter ursingii strain RIVM_C010559

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: LSO60_RS17415
• Protein ID: WP_004647749.1
• Coordinates: 249414..249710 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LSO60_RS17420
• Protein ID: WP_004969304.1
• Coordinates: 249712..250002 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSO60_RS17390 (LSO60_17355)	245364..245555	-	192	WP_228719710.1	hypothetical protein	-
LSO60_RS17395 (LSO60_17360)	245770..246378	-	609	WP_228719709.1	nucleotidyltransferase	-
LSO60_RS17400 (LSO60_17365)	247110..247676	+	567	WP_043041640.1	recombinase family protein	-
LSO60_RS17405 (LSO60_17370)	247897..248970	+	1074	WP_043041639.1	hypothetical protein	-
LSO60_RS17410	249073..249198	-	126	WP_004969301.1	hypothetical protein	-
LSO60_RS17415 (LSO60_17375)	249414..249710	+	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSO60_RS17420 (LSO60_17380)	249712..250002	+	291	WP_004969304.1	putative addiction module antidote protein	Antitoxin
LSO60_RS17425 (LSO60_17385)	250099..251424	-	1326	WP_081313053.1	ISNCY family transposase	-
LSO60_RS17430 (LSO60_17390)	251576..252454	-	879	WP_004973252.1	IS982-like element ISAba4 family transposase	-
LSO60_RS17435 (LSO60_17395)	252584..254038	-	1455	WP_026438532.1	SulP family inorganic anion transporter	-
LSO60_RS17440 (LSO60_17400)	254288..254497	-	210	WP_004976362.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / blaCARB-2 / sul1 / dfrA19 / blaIMP-4 / catB3 / ARR-3 / qacE / aph(3')-VIa / msr(E) / mph(E) / blaOXA-58 / aac(3)-IId	-	1..313387	313387
	-	inside	IScluster/Tn	msr(E) / mph(E)	-	250099..264257	14158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T227563 WP_004647749.1 NZ_CP089052:249414-249710 [Acinetobacter ursingii]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T227563 NZ_CP122951:579124-579390 [Helicobacter pylori]
GTGTTGAAACTCAACCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTACTTTTGAATGGGTTTGATGATAGCGTTTT
GAATAAGGTCATTCTGTCTTTGAGAAAAAAAGAACCGCTAGACCCACAATTTCAAGATCATGCTTTAAAGGGAAAGTGGA
AACCTTTTAGGGAATGCCACATCAAAGCTGATATTTTGCTTGTGTATTTGGTGAAAGATGATGAGCTTATTTTAGTAAGA
CTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10524.19 Da
• Isoelectric Point: 5.1133

>AT227563 WP_004969304.1 NZ_CP089052:249712-250002 [Acinetobacter ursingii]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT227563 NZ_CP122951:578766-579143 [Helicobacter pylori]
ATGCCTAACTCTCCCACCAAAAAAGACTACACACGATACAGCGAAAAACAGCTTTTTAACTTAATCAATCAATTAGAGCG
AAAAATCAAAAAGATGCAAAATGATAGAACTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCATTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGCTTAACAAAGAAACCGATTTGATTGTTGAGGA
TTTTTCTAGTTATAGCGATGAAAGAAAAAGGGCGTTAGGTGTTGAAACTCAACCTTAA